Abstract | BACKGROUND: MATERIAL/METHODS: Three human bladder transitional cancer cell lines - T24, 647V and SW780 - were treated with TRAIL and/or chlorin-based PDT. The cytotoxicity was measured by MTT and LDH assays and apoptosis was detected using annexin V by flow cytometry. RESULTS: CONCLUSIONS:
PDT with Ce6-PVP photosensitizer enhances the cytotoxic and apoptotic effects of TRAIL on bladder cancer cells. The obtained results suggest that combined treatment by TRAIL and PDT may provide the basis for a new therapeutic approach to induce cell death in bladder cancer.
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Authors | Ewelina Szliszka, Zenon P Czuba, Aleksandra Kawczyk-Krupka, Karolina Sieron-Stoltny, Aleksander Sieron, Wojciech Krol |
Journal | Medical science monitor : international medical journal of experimental and clinical research
(Med Sci Monit)
Vol. 18
Issue 1
Pg. BR47-53
(Jan 2012)
ISSN: 1643-3750 [Electronic] United States |
PMID | 22207109
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Annexin A5
- Porphyrins
- TNF-Related Apoptosis-Inducing Ligand
- Tetrazolium Salts
- Thiazoles
- chlorin
- L-Lactate Dehydrogenase
- thiazolyl blue
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Topics |
- Analysis of Variance
- Annexin A5
- Apoptosis
(drug effects)
- Cell Line, Tumor
- Flow Cytometry
- Humans
- L-Lactate Dehydrogenase
(metabolism)
- Photochemotherapy
(methods)
- Porphyrins
(pharmacology, therapeutic use)
- Statistics, Nonparametric
- TNF-Related Apoptosis-Inducing Ligand
(pharmacology, therapeutic use)
- Tetrazolium Salts
- Thiazoles
- Urinary Bladder Neoplasms
(drug therapy)
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