Cross-reactivity of Fusarium spp. in the Aspergillus Galactomannan enzyme-linked immunosorbent assay.

Abstract

Nine of 11 hematological patients with disseminated/deep-seated Fusarium infection tested at least twice for Aspergillus galactomannan (GM) had repeated positive results in the absence of Aspergillus isolation in culture. The centrifuged supernatants of 12 Fusarium isolates were tested by a GM enzyme-linked immunosorbent assay (EIA). All the isolates produced positive reactions when tested undiluted. These results show cross-reactivity of Fusarium spp. with Aspergillus GM that may constitute a drawback with respect to the specificity of the Platelia EIA.

• Authors: Anna Maria Tortorano, Maria Carmela Esposto, Anna Prigitano, Anna Grancini, Cristina Ossi, Caterina Cavanna, Giuliana Lo Cascio
• Journal: Journal of clinical microbiology (J Clin Microbiol) Vol. 50 Issue 3 Pg. 1051-3 (Mar 2012) ISSN: 1098-660X [Electronic] United States
• PMID: 22205818 (Publication Type: Journal Article)

Chemical References

• Mannans
• galactomannan
• Galactose

Topics

• Adult
• Aspergillosis (diagnosis, microbiology)
• Aspergillus (isolation & purification)
• Child
• Child, Preschool
• Clinical Laboratory Techniques (methods)
• Cross Reactions
• Enzyme-Linked Immunosorbent Assay (methods)
• Female
• Fusariosis (diagnosis, microbiology)
• Fusarium (isolation & purification)
• Galactose (analogs & derivatives)
• Humans
• Male
• Mannans (analysis)
• Middle Aged
• Young Adult