The efficiency of a urotensin II antagonist in an experimental lung fibrosis model.

Abstract

Pulmonary fibrosis is a chronic disease. Urotensin II (U-II) is a new peptide with angiogenic and profibrotic features. Therefore, we aim to evaluate the antagonism of U-II with palosuran in an animal model and plan to measure U-II, endothelin-1 (ET-1), and transforming growth factor-β1 (TGF-β1) and their association with lung fibrosis. Thirty Wistar male rats were used in the study and were divided into three groups: group 1, control; group 2, bleomycin-induced lung fibrosis group; and group 3, bleomycin-induced lung fibrosis with treatment palosuran group. U-II level (nanograms per milliliter) was 2.957 ± 0.159 in group1, 3.188 ± 0.122 in group 2, and 2.970 ± 0.165 in group 3 (p = 0.002). The ET-1 level (picograms per milliliter) was 4.486 ± 0.376 in group 1, 9.086 ± 1.850 in group 2, and 4.486 ± 0.376 in group 3 (p < 0.001). The TGF-β1 (nanograms per milliliter) level was 73.143 ± 9.96 in group 1, 84.81 ± 4.73 in group 2, and 77.86 ± 5.77 in group 3 (p = 0.006). Finally, the fibrosis score was 0.7 ± 0.48 in group 1, 4.4 ± 1.34 in group 2, and 3.2 ± 0.63 in group 3 (p < 0.001). There is a statistically significant positive relationship between fibrosis scores and the UT-II, ET-1, and TGF-β1 levels of the experimental lung fibrosis model. We believe U-II is an important mediator in lung fibrosis models, and its antagonism with palosuran could be a new treatment choice for interstitial lung fibrosis, but further studies need to be conducted to verify the findings of the current study.

• Authors: Ahmet Mesut Onat, Ibrahim Halil Turkbeyler, Yavuz Pehlivan, Tuncer Demir, Davut Sinan Kaplan, Seyithan Taysi, Ali Osman Ceribasi, Ediz Tutar, Bunyamin Kisacik
• Journal: Inflammation (Inflammation) Vol. 35 Issue 3 Pg. 1138-43 (Jun 2012) ISSN: 1573-2576 [Electronic] United States
• PMID: 22205238 (Publication Type: Journal Article)

Chemical References

• Endothelin-1
• Quinolines
• Transforming Growth Factor beta1
• Urotensins
• Bleomycin
• Urea
• urotensin II
• 1-(2-(4-benzyl-4-hydroxypiperidin-1-yl)ethyl)-3-(2-methylquinolin-4-yl)urea

Topics

• Animals
• Bleomycin
• Endothelin-1 (analysis)
• Lung (pathology)
• Male
• Pulmonary Fibrosis (chemically induced, drug therapy)
• Quinolines (pharmacology, therapeutic use)
• Random Allocation
• Rats
• Rats, Wistar
• Transforming Growth Factor beta1 (analysis)
• Urea (analogs & derivatives, pharmacology, therapeutic use)
• Urotensins (antagonists & inhibitors, metabolism)