Abstract | OBJECTIVE: ANIMALS: 101 client- and shelter-owned dogs. PROCEDURES: Study dogs included 74 clinically normal dogs, 17 dogs with neoplasia, and 10 dogs with renal insufficiency. For each dog, a blood sample was collected into an EDTA-containing tube; plasma U and UH(2) concentrations were determined via UV high-performance liquid chromatography, and the U:UH(2) concentration ratio was calculated. Data were compared among dogs grouped on the basis of sex, clinical group assignment, reproductive status (sexually intact, spayed, or castrated), and age. RESULTS: Mean ± SEM U:UH(2) concentration ratio for all dogs was 1.55 ± 0.08 (median, 1.38; range, 0.4 to 7.14). In 14 (13.9%) dogs, the U:UH(2) concentration ratio was considered abnormal (ie, > 2). Overall, mean ratio for sexually intact dogs was significantly higher than that for neutered dogs; a similar difference was apparent among males but not females. Dogs with ratios > 2 and dogs with ratios ≤ 2 did not differ significantly with regard to sex, clinical group, reproductive status, or age. CONCLUSIONS AND CLINICAL RELEVANCE: Determination of the U:UH(2) concentration ratio was easy to perform. Ratios were variable among dogs, possibly suggesting differences in dihydropyrimidine dehydrogenase activity. However, studies correlating U:UH(2) concentration ratio and fluoropyrimidine antimetabolite drug tolerability are required to further evaluate the test's validity and its appropriate use in dogs.
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Authors | Chad W Schmiedt, Corey F Saba, Kimberly G Freeman, Gaylen L Edwards |
Journal | American journal of veterinary research
(Am J Vet Res)
Vol. 73
Issue 1
Pg. 119-24
(Jan 2012)
ISSN: 1943-5681 [Electronic] United States |
PMID | 22204297
(Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- dihydrouracil
- Uracil
- Dihydrouracil Dehydrogenase (NADP)
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Topics |
- Animals
- Chromatography, High Pressure Liquid
(methods, veterinary)
- Dihydrouracil Dehydrogenase (NADP)
(metabolism)
- Dog Diseases
(blood, metabolism)
- Dogs
(blood, metabolism)
- Female
- Male
- Neoplasms
(blood)
- Renal Insufficiency
(blood)
- Uracil
(analogs & derivatives, blood)
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