Ambient particulate matter (PM) can increase the incidence of
arrhythmia. However, the arrhythmogenic mechanism of PM is poorly understood. This study investigated the arrhythmogenic mechanism of PM. In Sprague-Dawley rats, QT interval was increased from 115.0±14.0 to 142.1±18.4ms (p=0.02) after endotracheal exposure of
DEP (200μg/ml for 30min, n=5). Ventricular premature contractions were more frequently observed after
DEP exposure (100%) than baseline (20%, p=0.04). These effects were prevented by pretreatment of
N-acetylcysteine (NAC, 5mmol/L, n=3). In 12 Langendorff-perfused rat hearts,
DEP infusion of 12.5μg/ml for 20min prolonged action potential duration (APD) at only left ventricular base increasing apicobasal repolarization gradients. Spontaneous early afterdepolarization (EAD) and
ventricular tachycardia (VT) were observed in 8 (67%) and 6 (50%) hearts, respectively, versus no spontaneous triggered activity or VT in any hearts before
DEP infusion.
DEP-induced APD prolongation, EAD and VT were successfully prevented with NAC (5mmol/L, n=5),
nifedipine (10μmol/L, n=5), and active
Ca(2+)/calmodulin-dependent protein kinase II (
CaMKII) blockade,
KN 93 (1μmol/L, n=5), but not by
thapsigargin (200nmol/L) plus
ryanodine (10μmol/L, n=5) and inactive
CaMKII blockade,
KN 92 (1μmol/L, n=5). In neonatal rat cardiomyocytes,
DEP provoked ROS generation in dose dependant manner.
DEP (12.5μg/ml) induced apoptosis, and this effect was prevented by NAC and
KN 93. Thus, this study shows that in vivo and vitro exposure of PM induced APD prolongation, EAD and ventricular
arrhythmia. These effects might be caused by oxidative stress and
CaMKII activation.