Detection of fungal DNA in lysis-centrifugation blood culture for the diagnosis of invasive candidiasis in neonatal patients.

Abstract

We report data concerning the detection of fungal DNA directly from lysis-centrifugation blood culture to assess its value in the detection of fungaemia in 86 of the 347 patients admitted to the neonatal intensive-care unit between January 2009 and December 2010. The sensitivity and specificity of the PCR were 87.5% and 98.5%, respectively, with a positive predictive value of 93.3% and a negative predictive value of 97.1%. Detection of fungal DNA directly from blood culture Isolator 1.5 microbial tubes, without prior cultivation, is a promising approach for the rapid detection of Candida spp. in neonates with suspected candidaemia.

• Authors: L Trovato, P Betta, M G Romeo, S Oliveri
• Journal: Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases (Clin Microbiol Infect) Vol. 18 Issue 3 Pg. E63-5 (Mar 2012) ISSN: 1469-0691 [Electronic] England
• PMID: 22192484 (Publication Type: Evaluation Study, Journal Article)
• Copyright: © 2011 The Authors. Clinical Microbiology and Infection © 2011 European Society of Clinical Microbiology and Infectious Diseases.

Chemical References

• DNA, Fungal

Topics

• Blood (microbiology)
• Candida (genetics, isolation & purification)
• Candidiasis, Invasive (diagnosis)
• Centrifugation (methods)
• DNA, Fungal (genetics, isolation & purification)
• Fungemia (diagnosis)
• Humans
• Infant, Newborn
• Intensive Care Units, Neonatal
• Male
• Microbiological Techniques (methods)
• Molecular Diagnostic Techniques (methods)
• Sensitivity and Specificity