Evaluation of uptake and transport of ultrasmall superparamagnetic iron oxide nanoparticles by human brain-derived endothelial cells.

Abstract

AIM:

Ultrasmall superparamagnetic iron oxide nanoparticles (USPIO-NPs) are under development for imaging and drug delivery; however, their interaction with human blood-brain barrier models is not known.

MATERIALS & METHODS:

The uptake, reactive oxygen species production and transport of USPIO-NPs across human brain-derived endothelial cells as models of the blood-brain tumor barrier were evaluated for either uncoated, oleic acid-coated or polyvinylamine-coated USPIO-NPs.

RESULTS:

Reactive oxygen species production was observed for oleic acid-coated and polyvinylamine-coated USPIO-NPs. The uptake and intracellular localization of the iron oxide core of the USPIO-NPs was confirmed by transmission electron microscopy. However, while the uptake of these USPIO-NPs by cells was observed, they were neither released by nor transported across these cells even in the presence of an external dynamic magnetic field.

CONCLUSION:

USPIO-NP-loaded filopodia were observed to invade the polyester membrane, suggesting that they can be transported by migrating angiogenic brain-derived endothelial cells.

• Authors: Blanka Halamoda Kenzaoui, Catherine Chapuis Bernasconi, Heinrich Hofmann, Lucienne Juillerat-Jeanneret
• Journal: Nanomedicine (London, England) (Nanomedicine (Lond)) Vol. 7 Issue 1 Pg. 39-53 (Jan 2012) ISSN: 1748-6963 [Electronic] England
• PMID: 22191777 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Dextrans
• Magnetite Nanoparticles
• Polyvinyls
• Reactive Oxygen Species
• ferumoxtran-10
• polyvinylamine
• Oleic Acid

Topics

• Biological Transport
• Blood-Brain Barrier (physiology)
• Cell Survival
• Cells, Cultured
• Dextrans (administration & dosage, pharmacokinetics, radiation effects)
• Endothelial Cells (cytology, metabolism)
• Humans
• Magnetic Fields
• Magnetite Nanoparticles (administration & dosage, radiation effects)
• Microscopy, Electron, Transmission
• Oleic Acid (chemistry)
• Oxidative Stress
• Polyvinyls (chemistry)
• Reactive Oxygen Species (metabolism)