Supplementation of Nigella sativa fixed and essential oil mediates potassium bromate induced oxidative stress and multiple organ toxicity.

Abstract

The plants and their functional ingredients hold potential to cure various maladies and number of plants hold therapeutic potential. The present research was designed study the health promoting potential of black cumin (Nigella sativa) fixed oil (BCFO) and essential oil (BCEO) against oxidative stress with special reference to multiple organ toxicity. For the purpose, thirty rats (Strain: Sprague Dawley) were procured and divided into three groups (10 rats/group). The groups were fed on their respective diets i.e. D1 (control), D2 (BCFO @ 4.0%) and D3 (BCEO @ 0.30%) for a period of 56 days. Mild oxidative stress was induced with the help of potassium bromate injection @ 45 mg/Kg body weight. Furthermore, the levels of cardiac and liver enzymes were assayed. The results indicated that oxidative stress increased the activities of cardiac and liver enzymes. However, supplementation of BCFO and BCEO was effective in reducing the abnormal values of enzymes. Elevated levels of lactate dehydrogenase (LDH), CPK and CPK-MB were reduced from 456 to 231, 176 to 122 and 45 to 36mg/dL, respectively. Similarly, liver enzymes were also reduced. However, the results revealed that BCEO supplementation @ 0.30% is more effectual in ameliorating the multiple organ toxicity in oxidative stressed animal modelling. In the nutshell, it can be assumed that black cumin essential oil is more effective in reducing the extent of potassium bromate induced multiple organ toxicity (cardiac and liver enzymes imbalance) that will ultimately helpful in reducing the extent of myocardial and liver necrosis.

• Authors: Muhammad Tauseef Sultan, Masood Sadiq Butt, Rabia Shabeer Ahmad, Imran Pasha, Atif Nisar Ahmad, Mir Muhammad Nasir Qayyum
• Journal: Pakistan journal of pharmaceutical sciences (Pak J Pharm Sci) Vol. 25 Issue 1 Pg. 175-81 (Jan 2012) ISSN: 1011-601X [Print] Pakistan
• PMID: 22186327 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers
• Bromates
• Plant Oils
• potassium bromate
• Uric Acid
• Urea
• Creatine

Topics

• Animals
• Biomarkers (blood)
• Bromates
• Creatine (blood)
• Disease Models, Animal
• Drug Evaluation, Preclinical (methods, statistics & numerical data)
• Heart (drug effects)
• Humans
• Kidney (drug effects, metabolism)
• Multiple Organ Failure (chemically induced, diet therapy, enzymology)
• Myocardium (enzymology)
• Nigella sativa (chemistry)
• Oxidative Stress (drug effects)
• Phytotherapy (methods)
• Plant Oils (pharmacology, therapeutic use)
• Rats
• Rats, Sprague-Dawley
• Urea (blood)
• Uric Acid (blood)