The expression and anatomical distribution of BTLA and its ligand HVEM in rheumatoid synovium.

Abstract

Co-inhibitory signaling from B and T lymphocyte attenuator (BTLA) can suppress lymphocyte activation and maintain peripheral tolerance. However, the expression and anatomical distribution of BTLA and its ligand, herpesvirus entry mediator (HVEM), in rheumatoid arthritis (RA) synovium have not been reported. In this study, we analyzed the expression of HVEM and BTLA in RA synovium by immunohistochemistry, and our results showed that both factors were observed in all four cases of RA samples. At the cellular level, both HVEM and BTLA were found on the cell membrane and in the cytoplasm. Fluorescence dual staining demonstrated that HVEM was chiefly on CD3(+) T cells, CD68(+) macrophages, and to a lesser extent was found on CD31(+) endothelial cells. Similarly, the expression of BTLA was observed on infiltrated CD3(+) T cells and CD68(+) macrophages. The co-expression of HVEM and BTLA with some members of the B7 family in these sections was also analyzed, and the results showed that HVEM antigen was also found on B7-H3(+) capillaries, while it was absent on B7-H1(+), B7-DC(+), B7-H4(+), and Z39Ig(+) cells. Interestingly, BTLA was observed on B7-H1(+), B7-H4(+), and HVEM(+) cells in the synovium. The characteristic expression and distribution of BTLA/HVEM in the synovium indicated that their signaling probably affects the pathogenesis of RA, and a clear understanding of their functional roles may further elucidate the pathogenesis of this disease.

• Authors: Yongjun Shang, Guoning Guo, Qifu Cui, Jinlong Li, Zhihua Ruan, Yongwen Chen
• Journal: Inflammation (Inflammation) Vol. 35 Issue 3 Pg. 1102-12 (Jun 2012) ISSN: 1573-2576 [Electronic] United States
• PMID: 22179929 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, CD
• Antigens, Differentiation, Myelomonocytic
• B7 Antigens
• BTLA protein, human
• CD3 Complex
• CD68 antigen, human
• Platelet Endothelial Cell Adhesion Molecule-1
• Receptors, Immunologic
• Receptors, Tumor Necrosis Factor, Member 14
• TNFRSF14 protein, human

Topics

• Antigens, CD (biosynthesis)
• Antigens, Differentiation, Myelomonocytic (biosynthesis)
• Arthritis, Rheumatoid (immunology, metabolism, pathology)
• B7 Antigens (biosynthesis)
• CD3 Complex (biosynthesis)
• Endothelial Cells (immunology, metabolism)
• Humans
• Lymphocyte Activation
• Macrophages (immunology, metabolism)
• Platelet Endothelial Cell Adhesion Molecule-1 (biosynthesis)
• Receptors, Immunologic (metabolism)
• Receptors, Tumor Necrosis Factor, Member 14 (metabolism)
• Signal Transduction
• Synovial Membrane (metabolism)
• T-Lymphocytes (immunology, metabolism)