Spectroscopic and histological evaluation of wound healing progression following Low Level Laser Therapy (LLLT).

Abstract

The present study focuses on the evaluation of the effect of He-Ne laser on tissue regeneration by monitoring collagen synthesis in wound granulation tissues in Swiss albino mice using analysis of laser induced fluorescence (LIF) and light microscopy techniques. The spectral analyses of the wound granulation tissues have indicated a dose dependent increase in collagen levels during the post-wounding days. The histological examinations on the other hand have also shown a significant increase in collagen deposition along with the reduced edema, leukocytes, increased granulation tissue, and fibroblast number in the optimal laser dose treated group compared to the non-illuminated controls.

• Authors: Vijendra Prabhu, Satish B S Rao, Subhash Chandra, Pramod Kumar, Lakshmi Rao, Vasudeva Guddattu, Kapaettu Satyamoorthy, Krishna K Mahato
• Journal: Journal of biophotonics (J Biophotonics) Vol. 5 Issue 2 Pg. 168-84 (Feb 2012) ISSN: 1864-0648 [Electronic] Germany
• PMID: 22174176 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Chemical References

• Collagen

Topics

• Animals
• Biopsy
• Collagen (chemistry)
• Disease Progression
• Equipment Design
• Female
• Granulation Tissue (pathology)
• Inflammation
• Lasers
• Low-Level Light Therapy (methods)
• Male
• Mice
• Microscopy, Fluorescence (methods)
• Optics and Photonics (methods)
• Skin (pathology)
• Spectrophotometry (methods)
• Wound Healing (radiation effects)