Paris saponin I induces G₂/M cell cycle arrest and apoptosis in human gastric carcinoma SGC7901 cells.

Abstract

The aim of this study was to investigate the effect of Paris saponin I (PS I) on human gastric carcinoma cell growth and apoptosis and to explore the potential mechanisms. The proliferation of SGC7901 cells was monitored by the MTT cell viability assay, while the nuclear morphology of apoptotic cells was assessed by Hoechst 33258 staining. Flow cytometry was performed to analyze the cell cycle progression of propidium iodide (PI)-stained SGC7901 cells and the apoptotic rate of annexin V/PI-stained cells. Western blotting was used to examine the expression of several cell cycle proteins, including cyclin B1 and Cdk1, and the apoptosis-regulated proteins Bcl-2, Bax, cytochrome c, procaspase-9, and procaspase-3. The MTT assay demonstrated that PS I could induce significant dose- and time-dependent inhibition of SGC7901 cell proliferation. Marked morphological changes, including condensation of chromatin, nuclear fragmentation and apoptotic bodies were clearly shown on Hoechst 33258 staining. PSI treatment also resulted in the disruption of the cell cycle at G₂/M and the induction of apoptosis. Following PSI treatment, the cell cycle-related proteins cyclin B1 and Cdk1 were down-regulated. Expression of the pro-apoptotic protein Bax was increased, while anti-apoptotic protein Bcl-2 decreased. PSI treatment resulted in elevated cytoplasmic cytochrome c and activation of the apoptotic proteases caspase-9 and caspase-3. These data indicate that PS acts as an inhibitor of proli I feration in SGC7901 cells by inducing cell cycle arrest and mitochondria-dependent apoptosis. PSI is a potential therapeutic agent against human gastric carcinoma.

• Authors: Meifang Xiao, Xiahong Dai, Xinchun He, Rongrong Zhou, Baoxin Zhang, Guansheng Hu, Zebing Huang, Xuegong Fan
• Journal: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban (J Huazhong Univ Sci Technolog Med Sci) Vol. 31 Issue 6 Pg. 768-772 (Dec 2011) ISSN: 1672-0733 [Print] China
• PMID: 22173496 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents, Phytogenic
• Plant Extracts
• Saponins
• polyphyllin D
• Diosgenin

Topics

• Antineoplastic Agents, Phytogenic (pharmacology)
• Apoptosis (drug effects)
• Cell Cycle Checkpoints (drug effects)
• Cell Division
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Diosgenin (analogs & derivatives)
• G2 Phase
• Humans
• Liliaceae (chemistry)
• Plant Extracts (pharmacology)
• Saponins (pharmacology)
• Stomach Neoplasms (pathology)