The optimal strategy of noninvasive limb ischemic preconditioning for protecting heart against ischemia-reperfusion injury in rats.

Abstract

BACKGROUND:

Limb ischemic preconditioning (LIPC) induced by prior brief periods of ischemia-reperfusion (I/R) to a limb is a simple and convenient strategy to protect the heart from I/R injury. However, the optimal strategy is unknown. Therefore, the present study was conducted to test the most effective method of LIPC for clinical applications.

METHODS:

Male Wistar rats were randomized into the following groups: control groups, consecutive LIPC groups, intermittent LIPC groups. The control groups, including the sham operation group, the ischemia-reperfusion (I/R)-control group, the myocardial ischemic preconditioning (MIPC) group, the femoral artery ischemic preconditioning (FAIP) group; the consecutive LIPC groups, including continuously using for 1 d, 3 d, and 7 d groups. Each group was tested on the first, third, and fifth d after LIPC; intermittent LIPC groups, including 1-d LIPC + 1-d interval group, 1-d LIPC + 2-d interval group, 3-d LIPC + 3-d interval group, 3-d LIPC + 5-d interval group. Left ventricular function, incidence of ventricular arrhythmia, and ST-segment were measured during I/R. Myocardial infarct size, creatine kinase isoenzyme MB (CK-MB), and cardiac troponins I (cTnI) were determined at the end of experiment.

RESULTS:

Compared with the I/R-controls, the MIPC, FAIP, continuous LIPC for 3 and 7 d and 1-d LIPC + 1-d interval groups showed amelioration of ventricular arrhythmia, improved left ventricular function, lower ST-segment elevation, reduced myocardial infarct size, decreased CK-MB, and cTnI activity. The protective effects of LIPC persisted for 72 h.

CONCLUSIONS:

Our results suggest that a 1-d LIPC + 1-d interval provides optimal cardioprotection from I/R.

• Authors: Jianbo Gao, Yi Kang, Jianshi Lou
• Journal: The Journal of surgical research (J Surg Res) Vol. 174 Issue 2 Pg. e47-54 (May 15 2012) ISSN: 1095-8673 [Electronic] United States
• PMID: 22172137 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier Inc. All rights reserved.

Topics

• Animals
• Hindlimb (blood supply)
• Ischemic Preconditioning (methods)
• Male
• Random Allocation
• Rats
• Rats, Wistar
• Reperfusion Injury (prevention & control)