Regulation of wound-responsive calcium-dependent protein kinase from maize (ZmCPK11) by phosphatidic acid.

Abstract

In plant cells, phospholipids are not only membrane components but also act as second messengers interacting with various proteins and regulating diverse cellular processes, including stress signal transduction. Here, we report studies on the effects of various phospholipids on the activity and expression of maize wound-responsive calcium-dependent protein kinase (ZmCPK11). Our results revealed that in leaves treated with n-butanol, a potent inhibitor of phosphatidic acid (PA) synthesis catalyzed by phospholipase D, a significant decrease of ZmCPK11 activity was observed, indicating contribution of PA in the kinase activation. Using lipid binding assays, we demonstrate that among various phospholipids only saturated acyl species (16:0 and 18:0) of phosphatidic acid are able to bind to ZmCPK11. Saturated acyl species of PA are also able to stimulate phosphorylation of exogenous substrates by ZmCPK11 and autophosphorylation of the kinase. The level of ZmCPK11 autophosphorylation is correlated with its enzymatic activity. RT-PCR analysis showed that transcript level of ZmCPK11 in maize leaves increased in response to PA treatment. The influence of PA on the activity and transcript level of ZmCPK11 suggests an involvement of this kinase in a PA-mediated wound signal transduction pathway.

• Authors: Maria Klimecka, Jadwiga Szczegielniak, Luiza Godecka, Elżbieta Lewandowska-Gnatowska, Grażyna Dobrowolska, Grażyna Muszyńska
• Journal: Acta biochimica Polonica (Acta Biochim Pol) Vol. 58 Issue 4 Pg. 589-95 ( 2011) ISSN: 1734-154X [Electronic] Poland
• PMID: 22163350 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Phosphatidic Acids
• Plant Proteins
• RNA, Messenger
• Recombinant Proteins
• 1-Butanol
• Protein Kinases
• calcium-dependent protein kinase
• Phospholipase D

Topics

• 1-Butanol (pharmacology)
• Enzyme Activation
• Enzyme Assays
• Gene Expression Regulation, Plant
• Genes, Plant
• Mutagenesis, Site-Directed
• Phosphatidic Acids (metabolism, pharmacology)
• Phospholipase D (genetics, metabolism)
• Phosphorylation
• Plant Leaves (drug effects, genetics, metabolism)
• Plant Proteins (genetics, metabolism)
• Protein Binding
• Protein Kinases (genetics, metabolism)
• Protoplasts (cytology, metabolism)
• RNA, Messenger (analysis, genetics, metabolism)
• Recombinant Proteins (genetics, metabolism)
• Reverse Transcriptase Polymerase Chain Reaction
• Signal Transduction
• Stress, Physiological
• Time Factors
• Transfection
• Zea mays (drug effects, enzymology, genetics)