Processing and function of undermethylated chicken embryo fibroblast mRNA.

Abstract

Cycloleucine (1-aminocyclopentane-1-carboxylic acid) is a potent inhibitor of RNA methylation in B77 sarcoma virus-infected chicken embryo fibroblasts. Under conditions where 40 mM cycloleucine is present, internal N-6-methyladenosine and 5'-terminal cap 2'-O-ribose methylations of poly(A)+ RNA are inhibited greater than 90%. The methylation of the 5'-terminal 7-methylguanosine, however, does not appear to be significantly affected. The poly(A)+ RNA synthesized in cycloleucine-treated cells is transported from the nucleus to the cytoplasm and associates with polyribosomes at rates comparable to poly(A)+ RNA in untreated cells. On the other hand, the transport and utilization of newly synthesized ribosomal RNA in cycloleucine-treated cells is impaired, and the accumulation of mature 18 S and 28 S rRNA is reduced.

• Authors: K Dimock, C M Stoltzfus
• Journal: The Journal of biological chemistry (J Biol Chem) Vol. 254 Issue 13 Pg. 5591-4 (Jul 10 1979) ISSN: 0021-9258 [Print] United States
• PMID: 221474 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• RNA, Messenger
• Cycloleucine
• Poly A
• Methionine
• Uridine

Topics

• Animals
• Avian Sarcoma Viruses
• Cell Transformation, Viral
• Chick Embryo
• Cycloleucine (pharmacology)
• Fibroblasts (drug effects, metabolism)
• Kinetics
• Methionine (metabolism)
• Methylation
• Molecular Weight
• Poly A (metabolism)
• RNA, Messenger (metabolism)
• Uridine (metabolism)