Drought-responsive mechanisms in rice genotypes with contrasting drought tolerance during reproductive stage.

Abstract

Water status is the main factor affecting rice production. In order to understand rice strategies in response to drought condition in the field, the drought-responsive mechanisms at the physiological and molecular levels were studied in two rice genotypes with contrasting susceptibility to drought stress at reproductive stage. After 20 d of drought treatment, the osmotic potential of leaves reduced 78% and 8% in drought susceptible rice cultivar Zhenshan97B and tolerant rice cultivar IRAT109, respectively. The panicle lengths had no obvious changes in drought stressed Zhenshan97B and IRAT109, suggesting that drought stress impose less effect on assimilate translocation from leaf to vegetative growth of panicles. IRAT109 showed more extensive deeper root growth that could be considered a second line of defense against drought stress. The C(i)/C(a) ratio exhibited enhancement over reduction of g(s) in both cultivars, reflecting the non-stomatal limitation to photosynthesis occurred during drought stress. Orthophosphate dikinase, glycine dehydrogenase, ribulose bisphosphate carboxylase (Rubisco), glycine hydroxymethyltransferase and ATP synthase were down-regulated for Zhenshan97B in response to drought stress, suggesting the reduction of capacity of carbon assimilation in this rice cultivar. In drought-stressed IRAT109, transketolase, Rubisco were down-regulated, however, Rubisco activase and peptidyl-prolyl cis-trans isomerase, which might alleviate the damage on Rubisco by drought stress, were up-regulated. The increased abundances of chloroplastic superoxide dismutase [Cu-Zn] and dehydroascorbate reductase might provide antioxidant protection for IRAT109 against damage by dehydration.

• Authors: Kuixian Ji, Yangyang Wang, Weining Sun, Qiaojun Lou, Hanwei Mei, Shihua Shen, Hui Chen
• Journal: Journal of plant physiology (J Plant Physiol) Vol. 169 Issue 4 Pg. 336-44 (Mar 01 2012) ISSN: 1618-1328 [Electronic] Germany
• PMID: 22137606 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier GmbH. All rights reserved.

Chemical References

• Antioxidants
• Chloroplast Proteins
• Plant Proteins
• Water
• Ribulose-Bisphosphate Carboxylase

Topics

• Adaptation, Physiological (physiology)
• Antioxidants (metabolism)
• Chloroplast Proteins (metabolism)
• Chloroplasts (metabolism)
• Droughts
• Gene Expression Regulation, Plant (physiology)
• Genotype
• Oryza (enzymology, genetics, physiology)
• Osmotic Pressure
• Phenotype
• Photosynthesis (physiology)
• Plant Leaves (enzymology, genetics, physiology)
• Plant Proteins (metabolism)
• Plant Roots (genetics, metabolism)
• Plant Transpiration (physiology)
• Proteomics (methods)
• Reproduction
• Ribulose-Bisphosphate Carboxylase (genetics, metabolism)
• Stress, Physiological (physiology)
• Up-Regulation (physiology)
• Water (metabolism)