Humoral and cellular immune responses in pigs immunized intranasally with crude rhoptry proteins of Toxoplasma gondii plus Quil-A.

Abstract

We evaluated the humoral and cellular immune responses in pigs immunized intranasally with crude rhoptry proteins of Toxoplasma gondii plus Quil-A. The experiment used 13 mixed-breed pigs divided into the following three groups: G1 (vaccinated-challenged, n=6), which received the rhoptry vaccine (200(g/dose); G2 (adjuvant-challenged, n=4), which received PBS plus Quil-A; and G3 (unvaccinated-challenged, n=3), which was the control group. The treatments were performed intranasally at days 0, 21, and 42. Three pigs from G1 produced IgG and IgM antibody levels above the cut-off in the ELISA on the challenge day. Partial protection was observed in G1 at the chronic phase of infection when compared with G3. The preventable fractions were 41.6% and 6.5%, in G1 and G2, respectively. The results of this study suggest that rhoptry proteins plus Quil-A stimulated humoral, local, and systemic immune responses, which were able to partially protect the brain from cyst formation.

• Authors: Ivo Alexandre Leme da Cunha, Dauton Luiz Zulpo, Alexey Leon Gomel Bogado, Luiz Daniel de Barros, Alessandra Taroda, Michelle Igarashi, Italmar Teodorico Navarro, João Luis Garcia
• Journal: Veterinary parasitology (Vet Parasitol) Vol. 186 Issue 3-4 Pg. 216-21 (May 25 2012) ISSN: 1873-2550 [Electronic] Netherlands
• PMID: 22137347 (Publication Type: Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier B.V. All rights reserved.

Chemical References

• Adjuvants, Immunologic
• Immunoglobulin G
• Immunoglobulin M
• Protozoan Proteins
• Protozoan Vaccines
• Quillaja Saponins
• Saponins
• Quil A

Topics

• Adjuvants, Immunologic (administration & dosage)
• Administration, Intranasal
• Animals
• Brain (parasitology)
• Cell Proliferation
• Immunity, Cellular
• Immunity, Humoral
• Immunoglobulin G (blood, immunology)
• Immunoglobulin M (blood, immunology)
• Lymphocytes (physiology)
• Mice
• Protozoan Proteins (immunology)
• Protozoan Vaccines (administration & dosage, immunology)
• Quillaja Saponins
• Saponins (immunology)
• Swine
• Swine Diseases (parasitology, prevention & control)
• Toxoplasma (immunology, metabolism)
• Toxoplasmosis, Animal (prevention & control)