Monitoring honeybee venom immunotherapy in children with the basophil activation test.

New in vitro methods are essential for developing better follow-up criteria for venom immunotherapy (VIT).
Thirty-one children with a history of honeybee venom-induced systemic anaphylaxis were included in this prospective, single-blinded study. The basophil CD63 activation test (BAT) was assessed before starting VIT, at the end of the build-up phase (day 5), 6 months later, and after 2-4 yr of VIT.
Basophil CD63 activation test allowed identification of the culprit insect in 74% of honeybee venom-allergic children. In comparison, IgE reactivity was single positive in only 52% of children. Five days after starting VIT, BAT was highly comparable to before VIT. However, after 6 months and further after 2-4 yr of VIT, a significant and approximately fourfold decrease was demonstrated in CD63 response at sub-maximal 0.1 μg/ml allergen concentration, which mainly represents cellular sensitivity. No such differences were found at a higher 1 μg/ml of allergen concentration. Person-to-person analyses showed that after 2-4 yr of VIT, a marked CD63 decrease was evident in 85% of children. In addition, elevated basophil sensitivity measured before VIT was associated with the appearance of side effects observed during the build-up phase of VIT.
Basophil CD63 allergen-specific sensitivity seems to be a promising tool for monitoring protective immune response in honeybee VIT.
AuthorsSimona E K Žitnik, Tina Vesel, Tadej Avčin, Mira Šilar, Mitja Košnik, Peter Korošec
JournalPediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology (Pediatr Allergy Immunol) Vol. 23 Issue 2 Pg. 166-72 (Mar 2012) ISSN: 1399-3038 [Electronic] England
PMID22136583 (Publication Type: Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't)
Copyright© 2011 John Wiley & Sons A/S.
Chemical References
  • Bee Venoms
  • Adolescent
  • Anaphylaxis (etiology, immunology, prevention & control)
  • Animals
  • Basophil Degranulation Test (methods)
  • Bee Venoms (adverse effects, immunology)
  • Bees (immunology)
  • Child
  • Child, Preschool
  • Desensitization, Immunologic
  • Female
  • Humans
  • Insect Bites and Stings (immunology)
  • Male
  • Sensitivity and Specificity

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