Detection of mutant uromodulin in transgenic mouse harboring a mutant human UMOD gene.

Abstract

Uromodulin is the most abundant protein secreted in urine, and the mutated form of the uromodulin gene is associated with uromodulin-associated kidney disease (UAKD). Although uromodulin accumulates in the kidney of UAKD patients, it is unclear whether this is the wildtype or mutant form. In this study, we established a liquid chromatography (LC)-mass spectrometry/mass spectrometry (MS/MS)-based method for the detection of uromodulin mutants, using the C148W mutant as a target molecule. Membrane and cytosolic fractions of kidney samples from transgenic (Tg) mice expressing the C148W uromodulin mutant were shown to contain human uromodulin by western blotting, and mutant uromodulin with the C148W mutant sequence was observed by proteomic and selected reaction monitoring analyses. Our LC-MS/MS-based method is therefore useful for detection of mutant uromodulin that is undetectable by western blotting alone.

• Authors: Makoto Yasuda, Kiyoko Kaneko, Kiyoka Kaneko, Hiroka Hachisu, Noriko Yamaoka, Ken-ichi Mawatari, Kazuya Nakagomi, Norihiko Minoura, Makoto Hosoyamada
• Journal: Nucleosides, nucleotides & nucleic acids (Nucleosides Nucleotides Nucleic Acids) Vol. 30 Issue 12 Pg. 1284-9 (Dec 2011) ISSN: 1532-2335 [Electronic] United States
• PMID: 22132988 (Publication Type: Journal Article)

Chemical References

• Mutant Proteins
• Uromodulin

Topics

• Amino Acid Sequence
• Animals
• Blotting, Western
• Electrophoresis, Polyacrylamide Gel
• Humans
• Kidney (metabolism)
• Male
• Mass Spectrometry
• Mice
• Mice, Inbred C57BL
• Mice, Transgenic
• Molecular Sequence Data
• Mutant Proteins (chemistry, genetics, metabolism)
• Mutation (genetics)
• Uromodulin (chemistry, genetics, metabolism)