Combination effects of digalloylresveratrol with arabinofuranosylcytosine and difluorodeoxycytidine in human leukemia and pancreatic cancer cells.

Abstract

Digalloylresveratrol (DIG) is a newly synthesized agent aimed to combine the biological effects of the natural compounds, gallic acid and resveratrol, which both are free radical scavengers exhibiting anticancer activity. In this study, we investigated the effects of DIG on the growth of human HL-60 leukemia cells and on the colony formation of human BxPC-3 and PANC-1 pancreatic cancer cells. DIG was applied alone and in combination with arabinofuranosylcytosine (Ara-C) or difluorodeoxycytidine (dFdC), depending on the cell line employed. All IC(50) values observed were in the low micromolar range rendering DIG a promising antitumor compound in vitro. Considering the combination experiments, DIG yielded additive effects with Ara-C in HL-60 cells and-to a lesser extent-with dFdC in BxPC-3 and PANC-1 cells. Owing to our results, DIG may be further investigated in vitro and in animals.

• Authors: Philipp Saiko, Geraldine Graser, Sibylle Madlener, Simone Schwarz, Georg Krupitza, Walter Jaeger, Venkateswarlu Somepalli, Trimurtulu Golakoti, Monika Fritzer-Szekeres, Thomas Szekeres
• Journal: Nucleosides, nucleotides & nucleic acids (Nucleosides Nucleotides Nucleic Acids) Vol. 30 Issue 12 Pg. 1190-6 (Dec 2011) ISSN: 1532-2335 [Electronic] United States
• PMID: 22132974 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Stilbenes
• digalloylresveratrol
• Cytarabine
• Deoxycytidine
• Gallic Acid
• Gemcitabine

Topics

• Antineoplastic Combined Chemotherapy Protocols (pharmacology, therapeutic use)
• Cell Proliferation (drug effects)
• Cytarabine (chemistry, pharmacology, therapeutic use)
• Deoxycytidine (analogs & derivatives, chemistry, pharmacology, therapeutic use)
• Gallic Acid (analogs & derivatives, chemistry, pharmacology, therapeutic use)
• HL-60 Cells
• Humans
• Leukemia (drug therapy, pathology)
• Pancreatic Neoplasms (drug therapy, pathology)
• Stilbenes (chemistry, pharmacology, therapeutic use)
• Tumor Stem Cell Assay
• Gemcitabine