Biocompatibility of Fe₃O₄@Au composite magnetic nanoparticles in vitro and in vivo.

Abstract

PURPOSE:

This research was conducted to assess the biocompatibility of the core-shell Fe(3)O(4)@ Au composite magnetic nanoparticles (MNPs), which have potential application in tumor hyperthermia.

METHODS:

Fe(3)O(4)@Au composite MNPs with core-shell structure were synthesized by reduction of Au(3+) in the presence of Fe(3)O(4)-MNPs prepared by improved co-precipitation. Cytotoxicity assay, hemolysis test, micronucleus (MN) assay, and detection of acute toxicity in mice and beagle dogs were then carried out.

RESULTS:

The result of cytotoxicity assay showed that the toxicity grade of this material on mouse fibroblast cell line (L-929) was classified as grade 1, which belongs to no cytotoxicity. Hemolysis rates showed 0.278%, 0.232%, and 0.197%, far less than 5%, after treatment with different concentrations of Fe(3)O(4)@Au composite MNPs. In the MN assay, there was no significant difference in MN formation rates between the experimental groups and negative control (P > 0.05), but there was a significant difference between the experimental groups and the positive control (P < 0.05). The median lethal dose of the Fe(3)O(4)@Au composite MNPs after intraperitoneal administration in mice was 8.39 g/kg, and the 95% confidence interval was 6.58-10.72 g/kg, suggesting that these nanoparticles have a wide safety margin. Acute toxicity testing in beagle dogs also showed no significant difference in body weight between the treatment groups at 1, 2, 3, and 4 weeks after liver injection and no behavioral changes. Furthermore, blood parameters, autopsy, and histopathological studies in the experimental group showed no significant difference compared with the control group.

CONCLUSION:

The results indicate that Fe(3)O(4)@Au composite MNPs appear to be highly biocompatible and safe nanoparticles that are suitable for further application in tumor hyperthermia.

• Authors: Yuntao Li, Jing Liu, Yuejiao Zhong, Jia Zhang, Ziyu Wang, Li Wang, Yanli An, Mei Lin, Zhiqiang Gao, Dongsheng Zhang
• Journal: International journal of nanomedicine (Int J Nanomedicine) Vol. 6 Pg. 2805-19 ( 2011) ISSN: 1178-2013 [Electronic] New Zealand
• PMID: 22131827 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biocompatible Materials
• Magnetite Nanoparticles
• Gold

Topics

• Animals
• Biocompatible Materials (administration & dosage, toxicity)
• Body Weight (drug effects)
• Cell Line, Tumor
• Dogs
• Female
• Gold (administration & dosage, toxicity)
• Hemolysis
• Histocytochemistry
• Hyperthermia, Induced (adverse effects)
• Kidney (drug effects)
• Kidney Function Tests
• Lethal Dose 50
• Liver (drug effects)
• Liver Function Tests
• Magnetite Nanoparticles (administration & dosage, toxicity)
• Male
• Materials Testing
• Mice
• Micronucleus Tests
• Toxicity Tests, Acute