Abstract | BACKGROUND: MATERIALS AND METHODS: Cultured rat neonatal cardiomyocytes were subjected to hypoxia, and the expression of myocardin and ROS were evaluated. Different signal transduction inhibitors, atorvastatin and N-acetylcysteine (NAC) were used to identify the pathways that inhibited myocardin expression and ROS. Electrophoretic motility shift assay (EMSA) and luciferase assay were used to identify the binding of myocardin/ serum response factor (SRF) and transcription to cardiomyocytes. Cardiomyocyte hypertrophy was assessed by (3)H-proline incorporation assay. RESULTS: CONCLUSIONS:
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Authors | Chiung-Zuan Chiu, Bao-Wei Wang, Kou-Gi Shyu |
Journal | European journal of clinical investigation
(Eur J Clin Invest)
Vol. 42
Issue 5
Pg. 564-71
(May 2012)
ISSN: 1365-2362 [Electronic] England |
PMID | 22129233
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2011 The Authors. European Journal of Clinical Investigation © 2011 Stichting European Society for Clinical Investigation Journal Foundation. |
Chemical References |
- Heptanoic Acids
- Hydroxymethylglutaryl-CoA Reductase Inhibitors
- Nuclear Proteins
- Pyrroles
- Reactive Oxygen Species
- Trans-Activators
- myocardin
- Atorvastatin
- Extracellular Signal-Regulated MAP Kinases
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Topics |
- Animals
- Animals, Newborn
- Atorvastatin
- Blotting, Western
- Cells, Cultured
- Electrophoretic Mobility Shift Assay
- Extracellular Signal-Regulated MAP Kinases
(metabolism)
- Heptanoic Acids
(pharmacology)
- Hydroxymethylglutaryl-CoA Reductase Inhibitors
(pharmacology)
- Hypertrophy
(metabolism)
- Hypoxia
(metabolism, prevention & control)
- Myocytes, Cardiac
(drug effects, metabolism)
- Nuclear Proteins
(metabolism)
- Pyrroles
(pharmacology)
- Rats
- Rats, Wistar
- Reactive Oxygen Species
(metabolism)
- Real-Time Polymerase Chain Reaction
- Trans-Activators
(metabolism)
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