Abstract | BACKGROUND: METHODS: Eleven women with chronic neck-/ shoulder pain and eleven healthy women participated in this study. All participants went through microdialysis procedures in the trapezius muscle. Muscle dialysate samples were collected during four hours and analysed by nano liquid chromatography tandem mass spectrometry (nLC-MS/MS). RESULTS: We were able to detect AEA, PEA, N-stearoylethanolamine (SEA) and 2-arachidonoylglycerol (2-AG) in a single chromatographic run. Of the NAEs studied, PEA and SEA were clearly detectable in the muscle microdialysate samples. The muscle dialysate levels of PEA and SEA were significantly higher in myalgic subjects compared to healthy controls. CONCLUSION: This study demonstrates that microdialysis in combination with mass spectrometry can be used for analysing NAE's in human muscle tissue regularly over time. Furthermore the significant group differences in the concentration of PEA and SEA in this study might fill an important gap in our knowledge of mechanisms in chronic myalgia in humans. In the long run this expanded understanding of nociceptive and anitinociceptive processes in the muscle may provide a base for ameliorating treatment and rehabilitation of pain.
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Authors | Nazdar Ghafouri, Bijar Ghafouri, Britt Larsson, Maria V Turkina, Linn Karlsson, Christopher J Fowler, Björn Gerdle |
Journal | PloS one
(PLoS One)
Vol. 6
Issue 11
Pg. e27257
( 2011)
ISSN: 1932-6203 [Electronic] United States |
PMID | 22125609
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Amides
- Dialysis Solutions
- Endocannabinoids
- Ethanolamines
- Palmitic Acids
- Stearic Acids
- stearoylethanolamide
- palmidrol
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Topics |
- Adult
- Amides
- Chromatography, Liquid
(methods)
- Chronic Pain
(etiology, metabolism)
- Dialysis Solutions
(chemistry)
- Endocannabinoids
- Ethanolamines
- Female
- Humans
- Microdialysis
- Middle Aged
- Muscle, Skeletal
(metabolism, pathology)
- Muscular Diseases
(complications, metabolism)
- Nanotechnology
(methods)
- Neck Pain
(etiology, metabolism)
- Palmitic Acids
(analysis, metabolism)
- Shoulder Pain
(etiology, metabolism)
- Stearic Acids
(analysis, metabolism)
- Tandem Mass Spectrometry
(methods)
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