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Two-step, PCR-free telomerase detection by using exonuclease III-aided target recycling.

Abstract
We report the sensitive detection of telomerase activity by using exonuclease III-aided target recycling to amplify the signal produced by a chimeric LNA-DNA molecular beacon. We demonstrate the specific detection of as few as 30 telomerase-positive breast cancer cells in a single-measurement fluorescence assay that avoids the problematic PCR and gel analysis of the current "gold-standard" assay.
AuthorsXiaolei Zuo, Fan Xia, Adriana Patterson, H Tom Soh, Yi Xiao, Kevin W Plaxco
JournalChembiochem : a European journal of chemical biology (Chembiochem) Vol. 12 Issue 18 Pg. 2745-7 (Dec 16 2011) ISSN: 1439-7633 [Electronic] Germany
PMID22125097 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
CopyrightCopyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Chemical References
  • DNA Probes
  • Telomerase
  • Exodeoxyribonucleases
  • exodeoxyribonuclease III
Topics
  • Base Sequence
  • Cell Line
  • Chromatography, High Pressure Liquid
  • DNA Probes
  • Exodeoxyribonucleases (metabolism)
  • Humans
  • Mass Spectrometry
  • Polymerase Chain Reaction (methods)
  • Telomerase (metabolism)

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