Abstract |
We report the sensitive detection of telomerase activity by using exonuclease III-aided target recycling to amplify the signal produced by a chimeric LNA- DNA molecular beacon. We demonstrate the specific detection of as few as 30 telomerase-positive breast cancer cells in a single-measurement fluorescence assay that avoids the problematic PCR and gel analysis of the current "gold-standard" assay.
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Authors | Xiaolei Zuo, Fan Xia, Adriana Patterson, H Tom Soh, Yi Xiao, Kevin W Plaxco |
Journal | Chembiochem : a European journal of chemical biology
(Chembiochem)
Vol. 12
Issue 18
Pg. 2745-7
(Dec 16 2011)
ISSN: 1439-7633 [Electronic] Germany |
PMID | 22125097
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
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Copyright | Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Chemical References |
- DNA Probes
- Telomerase
- Exodeoxyribonucleases
- exodeoxyribonuclease III
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Topics |
- Base Sequence
- Cell Line
- Chromatography, High Pressure Liquid
- DNA Probes
- Exodeoxyribonucleases
(metabolism)
- Humans
- Mass Spectrometry
- Polymerase Chain Reaction
(methods)
- Telomerase
(metabolism)
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