Abstract |
The aim of this study is to explore human leukocyte antigen ( HLA)-DQ variability in two populations (Cayapas Amerindians and Afro-Ecuadorians) who live near one another along the Cayapa River and who are exposed to the same environmental stresses, such as infection by Onchocerca volvulus. HLA-DQA1 and HLA-DQB1 of 149 unrelated individuals (74 Cayapas and 75 Afro-Ecuadorians) have been analyzed. HLA high-resolution molecular typing was performed by sequence-based typing, sequence-specific oligonucleotides hybridization and sequence-specific primer (SSP) amplification. The comparison between affected (cases) and unaffected people (controls) in both populations shows the key role of several HLA-DQA1 alleles in susceptibility and protection against onchocerciasis. In both populations, there is strong evidence related to the protective role of DQA1*0401 against onchocerciasis. Alleles HLA-DQA1*0102 and *0103 seem to represent risk factors in Afro-Ecuadorians, while HLA-DQA1*0301 is only a suggestive susceptibility allele in Cayapas. These findings represent new positive/negative associations with onchocerciasis in South America, whereas previous findings pertained only to African populations.
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Authors | F De Angelis, A Garzoli, A Battistini, A Iorio, G F De Stefano |
Journal | Tissue antigens
(Tissue Antigens)
Vol. 79
Issue 2
Pg. 123-9
(Feb 2012)
ISSN: 1399-0039 [Electronic] England |
PMID | 22117902
(Publication Type: Journal Article)
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Copyright | © 2011 John Wiley & Sons A/S. |
Chemical References |
- DNA Primers
- HLA-DQ alpha-Chains
- HLA-DQ beta-Chains
- HLA-DQA1 antigen
- HLA-DQB1 antigen
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Topics |
- Adolescent
- Adult
- Aged
- Alleles
- American Indian or Alaska Native
- Animals
- Black People
- Case-Control Studies
- Child
- DNA Primers
- Disease Susceptibility
- Ecuador
(epidemiology)
- Female
- Gene Frequency
- Gene-Environment Interaction
- HLA-DQ alpha-Chains
(genetics, immunology)
- HLA-DQ beta-Chains
(genetics, immunology)
- Haplotypes
- Histocompatibility Testing
- Humans
- Male
- Middle Aged
- Nucleic Acid Amplification Techniques
- Onchocerca
(immunology)
- Onchocerciasis
(ethnology, genetics, immunology)
- Polymorphism, Genetic
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