Characterization of recombinant tick anticoagulant peptide. A highly selective inhibitor of blood coagulation factor Xa.

• Abstract: Tick anticoagulant peptide (TAP) is a potent, highly selective inhibitor of blood coagulation factor Xa (Waxman, L., Smith, D. E., Arcuri, K. E., and Vlasuk, G. P. (1990) Science, 248, 593-596). Further detailed studies pertaining to the in vitro and in vivo evaluation of TAP require quantities of the inhibitor which cannot be isolated from ticks. To overcome this limitation we describe here the characterization of recombinant TAP (rTAP) secreted by Saccharomyces cerevisiae. Expression of rTAP was obtained using a chimeric gene containing a fusion between sequences encoding the secretory preproleader of the yeast mating pheromone alpha-factor and a synthetic sequence encoding the 60-amino acid inhibitor under the transcriptional control of a galactose-inducible promoter. Recombinant S. cerevisiae were found to secrete biologically active rTAP into the extracellular medium at levels of 0.1-0.15 g/liter. The secreted inhibitor was purified to homogeneity and found to be indistinguishable from the native inhibitor with respect to several criteria, including primary structure, amino acid composition, and electrophoretic mobility. In addition, purified rTAP and native TAP exhibited similar stoichiometric inhibition of factor Xa in vitro. The in vivo efficacy of rTAP was demonstrated using a model of low grade disseminated intravascular coagulation where the purified inhibitor was shown to significantly inhibit thromboplastin-induced fibrinopeptide A generation following an infusion into conscious rhesus monkeys. The availability of rTAP will allow a detailed evaluation of the in vitro and in vivo properties of this highly specific and potent factor Xa inhibitor.
• Authors: M P Neeper, L Waxman, D E Smith, C A Schulman, M Sardana, R W Ellis, L W Schaffer, P K Siegl, G P Vlasuk (Affiliation: Department of Biological Chemistry, Merck Sharp & Dohme Research Laboratories, West Point, Pennsylvania 19486.)
• Journal: The Journal of biological chemistry (J Biol Chem) Vol. 265 Issue 29 Pg. 17746-52 (Oct 15 1990) ISSN: 0021-9258 [Print] UNITED STATES
• PMID: 2211658 (Publication Type: Journal Article)

Chemical References

• Oligonucleotide Probes
• Peptide Fragments
• Peptides
• Recombinant Proteins
• tick anticoagulant peptide
• Factor Xa

Topics

• Amino Acid Sequence
• Animals
• Base Sequence
• Factor Xa (antagonists & inhibitors)
• Genes, Synthetic
• Humans
• Molecular Sequence Data
• Oligonucleotide Probes
• Peptide Fragments (isolation & purification)
• Peptides (genetics, isolation & purification, pharmacology)
• Plasmids
• Polymerase Chain Reaction
• Recombinant Proteins (isolation & purification, pharmacology)
• Restriction Mapping
• Saccharomyces cerevisiae (genetics)
• Ticks