Abstract | BACKGROUND: Brain stem cardiovascular regulatory dysfunction during brain death is underpinned by an upregulation of nitric oxide synthase II ( NOS II) in rostral ventrolateral medulla (RVLM), the origin of a life-and-death signal detected from blood pressure of comatose patients that disappears before brain death ensues. Furthermore, the ubiquitin- proteasome system (UPS) may be involved in the synthesis and degradation of NOS II. We assessed the hypothesis that the UPS participates in brain stem cardiovascular regulation during brain death by engaging in both synthesis and degradation of NOS II in RVLM. METHODOLOGY/PRINCIPAL FINDINGS: CONCLUSIONS/SIGNIFICANCE: We conclude that UPS participates in the defunct and maintained brain stem cardiovascular regulation during experimental brain death by engaging in both synthesis and degradation of NOS II at RVLM. Our results provide information on new therapeutic initiatives against this fatal eventuality.
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Authors | Carol H Y Wu, Julie Y H Chan, Samuel H H Chan, Alice Y W Chang |
Journal | PloS one
(PLoS One)
Vol. 6
Issue 11
Pg. e27404
( 2011)
ISSN: 1932-6203 [Electronic] United States |
PMID | 22110641
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- RNA, Messenger
- Ubiquitin
- Nitric Oxide Synthase Type II
- Proteasome Endopeptidase Complex
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Topics |
- Animals
- Brain Death
(metabolism, physiopathology)
- Brain Stem
(enzymology, metabolism, physiopathology)
- Cardiovascular System
(physiopathology)
- Endotoxemia
(complications)
- Male
- Nitric Oxide Synthase Type II
(biosynthesis, genetics, metabolism)
- Proteasome Endopeptidase Complex
(metabolism)
- Proteolysis
- RNA, Messenger
(genetics, metabolism)
- Rats
- Rats, Sprague-Dawley
- Ubiquitin
(metabolism)
- Ubiquitination
- Up-Regulation
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