Abstract | OBJECTIVE: METHODS: Employing a globally proteomic strategy combining of immunoprecipitation, LC-MS/MS and SCX-LC-MS analysis to identified ubiquitination sites, which were identified by detecting signature peptides containing a GG-tag (114.1 Da) and an LRGG-tag (383.2 Da). RESULTS: In total, 52 ubiquitinated proteins containing 73 ubiquitination sites of which 14 and 59 sites contained LRGG-tag and GG-tag were identified, respectively. CONCLUSION: Classification analysis by of the proteins identified in the study based on the PANTHER showed that they were associated with multiple functional groups. This suggested the involvement of many endogenous proteins in the ubiquitination in MM.
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Authors | HongLing Jia, ChaoWu Liu, Feng Ge, ChuanLe Xiao, ChunHua Lu, Tong Wang, QingYu He |
Journal | Biomedical and environmental sciences : BES
(Biomed Environ Sci)
Vol. 24
Issue 4
Pg. 422-30
(Aug 2011)
ISSN: 0895-3988 [Print] China |
PMID | 22108332
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2011 The Editorial Board of Biomedical and Environmental Sciences. Published by Elsevier B.V. All rights reserved. |
Chemical References |
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Topics |
- Cell Line, Tumor
- Gene Expression Profiling
- Gene Expression Regulation, Neoplastic
(physiology)
- Humans
- Multiple Myeloma
(metabolism)
- Neoplasm Proteins
(genetics, metabolism)
- Proteomics
(methods)
- Ubiquitination
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