Abstract | BACKGROUND AND AIMS: METHODS: We conducted a cross-sectional, analytical study in 48 children with KD, 22 with CA. Control samples were obtained from 61 aged-matched children. We took a peripheral blood sample and extracted genomic DNA from all children participating in the study. Using restriction factor length polymorphism-polymerase chain reaction (RFLP-PCR), we performed determination of TNF -308 and LTA +252. RESULTS: There was no difference in frequency between the study groups for genotype LTA +252 (OR 0.37, 95% CI, 0.06-2, p = 0.44) or between groups for KD with or without coronary aneurysms for both polymorphisms. In subjects with KD, we did not observe the heterozygous genotype of TNF -308, the difference being significant (OR 12, 95% CI, 4.8-30.4, p = 0.0001) using the χ(2) test with the continuity correction on comparison with the control group. CONCLUSIONS: Comparative analysis by group did not show a significant difference in the frequency of the alleles and genotypes between KD with CA vs. KD without CA vs. controls, for both TNF -308 and LTA +252.
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Authors | Francisco Cruz-Olivo, Guadalupe García-Elorriaga, César González-Bonilla, Guillermo Del Rey-Pineda, Javier Mancilla-Ramírez |
Journal | Archives of medical research
(Arch Med Res)
Vol. 42
Issue 7
Pg. 602-7
(Oct 2011)
ISSN: 1873-5487 [Electronic] United States |
PMID | 22100609
(Publication Type: Journal Article)
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Copyright | Copyright © 2011 IMSS. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- Lymphotoxin-alpha
- Tumor Necrosis Factor-alpha
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Topics |
- Adolescent
- Child
- Child, Preschool
- Coronary Aneurysm
(diagnostic imaging, genetics)
- Cross-Sectional Studies
- Humans
- Infant
- Infant, Newborn
- Lymphotoxin-alpha
(genetics)
- Mexico
- Mucocutaneous Lymph Node Syndrome
(genetics)
- Polymerase Chain Reaction
- Polymorphism, Genetic
- Polymorphism, Restriction Fragment Length
- Tumor Necrosis Factor-alpha
(genetics)
- Ultrasonography
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