Abstract | AIMS: MAIN METHODS: The isoform-specific expression of MT and HO genes was assessed (RT-PCR) in rat livers following 3-day bile duct ligation, 2-h lipopolysaccharide treatment (1mg/kg) or their combination, with or without GdCl₃ pretreatment (10 mg/kg, 24h before endotoxin). Lipid peroxidation, DNA damage and hepatic antioxidant enzyme activities were also assessed. KEY FINDINGS: All these challenges induced similar extents of DNA damage, whereas the lipid peroxidation increased only when endotoxemia was combined with biliary obstruction. The MT and HO mRNA levels displayed isoform-specific changes: those of MT-1 and HO-2 did not change appreciably, whereas those of MT-2 and HO-1 increased significantly in 2-h endotoxemia, with or without obstructive jaundice. Among the enzymes reflecting the endogenous protective mechanisms, the catalase and copper/ zinc- superoxide dismutase levels decreased, while that of Mn-SOD slightly increased. Interestingly, GdCl₃ alone induced lipid peroxidation, DNA damage and MT-2 expression. In response to GdCl₃, HO-1 induction was significantly lower in each model. SIGNIFICANCE: Despite its moderate hepatocellular toxicity, the ameliorated stress-induced hepatic reactions provided by GdCl₃ may contribute to its protective effects.
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Authors | Szabolcs Abrahám, Edit Hermesz, Andrea Szabó, Agnes Ferencz, Zsanett Jancsó, Erno Duda, Magdolna Abrahám, György Lázár, György Lázár Jr |
Journal | Life sciences
(Life Sci)
Vol. 90
Issue 3-4
Pg. 140-6
(Jan 16 2012)
ISSN: 1879-0631 [Electronic] Netherlands |
PMID | 22100509
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2011 Elsevier Inc. All rights reserved. |
Chemical References |
- Isoenzymes
- Mt2A protein, rat
- Metallothionein
- Heme Oxygenase (Decyclizing)
- Hmox1 protein, rat
- heme oxygenase-2
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Topics |
- Animals
- DNA Damage
- Endotoxemia
(enzymology, genetics, metabolism)
- Gene Expression Regulation, Enzymologic
- Heme Oxygenase (Decyclizing)
(biosynthesis, genetics)
- Isoenzymes
(biosynthesis)
- Jaundice, Obstructive
(enzymology, genetics, metabolism)
- Kupffer Cells
(enzymology, metabolism, pathology)
- Liver
(enzymology, metabolism)
- Male
- Metallothionein
(biosynthesis, genetics)
- Random Allocation
- Rats
- Rats, Wistar
- Up-Regulation
(physiology)
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