The Gram-positive bacterium Clavibacter michiganensis subsp. michiganensis (Cmm) causes wilt and canker disease of tomato (Solanum lycopersicum). Mechanisms of Cmm pathogenicity and tomato response to Cmm
infection are not well understood. To explore the interaction between Cmm and tomato, multidimensional
protein identification technology (MudPIT) and tandem mass spectrometry were used to analyze in vitro and in planta generated samples. The results show that during
infection Cmm senses the plant environment, transmits signals, induces, and then secretes multiple hydrolytic
enzymes, including
serine proteases of the Pat-1, Ppa, and Sbt familes, the CelA, XysA, and NagA glycosyl
hydrolases, and other cell wall-degrading
enzymes. Tomato induction of pathogenesis-related (PR)
proteins, LOX1, and other defense-related
proteins during
infection indicates that the plant senses the invading bacterium and mounts a basal defense response, although partial with some suppressed components including class III
peroxidases and a secreted
serine peptidase. The tomato
ethylene-synthesizing
enzyme ACC-oxidase was induced during
infection with the wild-type Cmm but not during
infection with an endophytic Cmm strain, identifying Cmm-triggered host synthesis of
ethylene as an important factor in disease symptom development. The proteomic data were also used to improve Cmm genome annotation, and thousands of Cmm gene models were confirmed or expanded.