Effects of 2-methoxyestradiol on proliferation, apoptosis and gene expression of cyclin B1 and c-Myc in esophageal carcinoma EC9706 cells.

Abstract

2-Methoxyestradiol (2-ME) is an endogenous metabolite of 17β-estradiol. In this study, we determined the antitumour activities of 2-ME on the well-differentiated EC9706 esophageal carcinoma cells in vitro. 2-ME had a strong antiproliferative effect on EC9706 cells and caused an increase in the population of apoptotic cells, detected by flow cytometry. A significant number of cells were blocked in the G(2)/M phase of the cell cycle. 2-ME-treated cells demonstrated an increase in cyclin B1 and c-Myc protein levels, as well as an increase in the percentage of G(2)/M phase. Their up-regulation may be involved in 2-ME-induced apoptosis and G(2)/M cell cycle arrest of the EC9706 cells, and it precedes the onset of apoptosis.

• Authors: Bin Du, Zhilan Zhao, Huibin Sun, Sijia Ma, Jianwen Jin, Zhenzhong Zhang
• Journal: Cell biochemistry and function (Cell Biochem Funct) Vol. 30 Issue 2 Pg. 158-65 (Mar 2012) ISSN: 1099-0844 [Electronic] England
• PMID: 22095881 (Publication Type: Journal Article)
• Copyright: Copyright © 2011 John Wiley & Sons, Ltd.

Chemical References

• Cyclin B1
• Proto-Oncogene Proteins c-myc
• Estradiol
• 2-Methoxyestradiol

Topics

• 2-Methoxyestradiol
• Apoptosis (drug effects)
• Carcinoma (drug therapy, genetics, metabolism, physiopathology)
• Cell Cycle (drug effects)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Cyclin B1 (genetics, metabolism)
• Esophageal Neoplasms (drug therapy, genetics, metabolism, physiopathology)
• Estradiol (analogs & derivatives, pharmacology)
• Gene Expression Regulation, Neoplastic (drug effects)
• Humans
• Proto-Oncogene Proteins c-myc (genetics, metabolism)