HOMEPRODUCTSSERVICESCOMPANYCONTACTFAQResearchDictionaryPharmaMobileSign Up FREE or Login

[Protection of penehyclidine hydrochloride on renal tissue injury induced by limb ischemia/reperfusion].

AbstractOBJECTIVE:
To evaluate the protection of penehyclidine hydrochloric postconditioning on HIF-1α (hypoxia-inducible factor-1α) in renal tissue injury induced by lower limb ischemia/reperfusion (I/R).
METHODS:
A total of 72 adult male Wistar rats weighing 230 - 250 g were randomly divided into 3 groups: control (group C), limb ischemia-reperfusion (group R/I) and penehyclidine hydrochloride postconditioning (group P). The animals were anesthetized by inhaling 2% isoflurane and blood flow of bilateral lower limbs was blocked with rubber bands for 3 h in groups P and R/I. In group P, penehyclidine hydrochloride 0.15 mg/kg was injected via caudal vein at 3 min pre-reperfusion. After sacrificing, their kidneys were removed at 3 h of ischemia and 1, 3, 6 h of reperfusion respectively. The blood urea nitrogen (BUN) and creatinine (Cr) were detected by colorimetric method, plasma tumor necrosis factor-α (TNF-α) by ELISA (enzyme-linked immunosorbent assay) and HIF-1α of renal tissue by immunohistochemistry. Renal pathological changes were observed under light microscope.
RESULTS:
Compared with group C, the serum levels of BUN and Cr increased while TNF-α and HIF-1α were up-regulated in groups I/R and P (P < 0.05). As compared with group I/R, the serum levels of BUN, Cr and MDA decreased while TNF-α and HIF-1α were down-regulated in group P. [at T2: (15.10 ± 1.88) mmol/L vs (19.46 ± 2.76) mmol/L, (113 ± 10) µmol/L vs (143 ± 11) µmol/L, (13.8 ± 1.7) nmol/g vs (15.5 ± 1.8) nmol/g, (53.1 ± 3.1) ng/L vs (53.9 ± 4.8) ng/L, 0.298 ± 0.015 vs 0.471 ± 0.032, all P < 0.05].
CONCLUSION:
Penehyclidine hydrochloride can down-regulate the expression of HIF-1α and attenuate the renal injury induced by lower limb I/R. And the mechanisms may be through inhibiting the inflammatory reactions, reducing the release of oxygen free radicals and improving the conditions of hypoxia and ischemia.
AuthorsXing Xue, Yu-fang Leng, Yue Zhang, Yan Zhang, Tao Wang, Yu-qing Kang
JournalZhonghua yi xue za zhi (Zhonghua Yi Xue Za Zhi) Vol. 91 Issue 26 Pg. 1866-9 (Jul 12 2011) ISSN: 0376-2491 [Print] China
PMID22093793 (Publication Type: English Abstract, Journal Article)
Chemical References
  • Hypoxia-Inducible Factor 1, alpha Subunit
  • Quinuclidines
  • Reactive Oxygen Species
  • penehyclidine
Topics
  • Animals
  • Hypoxia-Inducible Factor 1, alpha Subunit (metabolism)
  • Kidney (drug effects, pathology)
  • Male
  • Quinuclidines (pharmacology)
  • Rats
  • Rats, Wistar
  • Reactive Oxygen Species (metabolism)
  • Reperfusion Injury (metabolism, pathology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!


Choose Username:
Email:
Password:
Verify Password: