Apoptosis induced by ZnPcH1-based photodynamic therapy in Jurkat cells and HEL cells.

Abstract

Photodynamic therapy (PDT) can selectively and effectively kill tumor cells, and photosensitization is the key to these anti-tumor effects. In this study, we investigated the killing mechanisms of the photosensitizer ZnPcH1 (a mono-α-substituted zinc(II) phthalocyanine synthesized in China), in the acute lymphoid leukemia cell line Jurkat and the acute erythroleukemia cell line HEL. Results from acridine orange/ethidium bromide fluorescence staining, DNA gel electrophoresis, and Annexin-V(FITC/PI) double-stained flow cytometry analysis indicated that ZnPcH1-PDT induced apoptosis in Jurkat and HEL cells, with Jurkat cells being more sensitive. Following ZnPcH1-PDT treatment, upregulation of p53 and Bax, downregulation of HSP70, Bcl-2 and Akt, and inhibition of the phosphorylation of Akt and GSK3β were observed. Our results establish a theoretical basis for the application of ZnPcH1-PDT in the treatment of acute leukemia.

• Authors: Huifang Huang, Xiaoyan Zhao, Yuanzhong Chen, Rong Lu, Yong Wu
• Journal: International journal of hematology (Int J Hematol) Vol. 94 Issue 6 Pg. 539-44 (Dec 2011) ISSN: 1865-3774 [Electronic] Japan
• PMID: 22090287 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Annexin A5
• Apoptosis Regulatory Proteins
• HSP70 Heat-Shock Proteins
• Indoles
• Isoindoles
• Organometallic Compounds
• Photosensitizing Agents
• Zinc Compounds
• Zn(II)-phthalocyanine
• GSK3B protein, human
• Glycogen Synthase Kinase 3 beta
• Glycogen Synthase Kinase 3

Topics

• Annexin A5 (metabolism)
• Apoptosis (drug effects, genetics)
• Apoptosis Regulatory Proteins (genetics, metabolism)
• Cell Line, Tumor
• Gene Expression Regulation, Leukemic (drug effects)
• Glycogen Synthase Kinase 3 (genetics, metabolism)
• Glycogen Synthase Kinase 3 beta
• HSP70 Heat-Shock Proteins (genetics, metabolism)
• Humans
• Indoles (pharmacology)
• Isoindoles
• Jurkat Cells
• Organometallic Compounds (pharmacology)
• Photochemotherapy (methods)
• Photosensitizing Agents (pharmacology)
• Staining and Labeling
• Zinc Compounds