Studying the interactions of a platinum(II) 9-aminoacridine complex with proteins and oligonucleotides by ESI-TOF MS.

Abstract

The interaction of a novel Pt complex, [Pt(dmba)(N9-9AA)(PPh(3))](+)1 (dmba = N,N-dimethylbenzylamine-κN,κC; 9AA = 9-aminoacridine), which exhibits anti-tumor activity, with certain key proteins has been monitored by ESI-MS. Also, the interaction of 1 with a designed double-stranded oligonucleotide containing the GG motif has been followed by mass spectrometry as well as by fluorimetry. The results obtained show the low interaction of 1 with the considered proteins and the absence of covalent interaction with the oligonucleotides, but the fluorimetric data confirm the π-π interaction of 1 with the double-stranded DNA, which is probably the reason of the previously reported activity of 1 in several tumor cell lines.

• Authors: Katia G Samper, Consuelo Vicente, Venancio Rodríguez, Sílvia Atrian, Natalia Cutillas, Mercè Capdevila, José Ruiz, Òscar Palacios
• Journal: Dalton transactions (Cambridge, England : 2003) (Dalton Trans) Vol. 41 Issue 1 Pg. 300-6 (Jan 07 2012) ISSN: 1477-9234 [Electronic] England
• PMID: 22089173 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Coordination Complexes
• Oligonucleotides
• Proteins
• Platinum
• Aminacrine

Topics

• Aminacrine (chemistry)
• Antineoplastic Agents (chemical synthesis, chemistry, toxicity)
• Cell Line, Tumor
• Coordination Complexes (chemical synthesis, chemistry, toxicity)
• Humans
• Oligonucleotides (chemistry)
• Platinum (chemistry)
• Proteins (chemistry)
• Spectrometry, Mass, Electrospray Ionization