Mollicutes contamination: a new strategy for an effective rescue of cancer cell lines.

Abstract

Mollicutes contaminations of cellular models can have marked effects on gene expression and cell behaviour in vitro leading to the production of unreliable data, unsafe biopharmaceutical drugs or to the loss of cell culture itself. Fortunately, irreplaceable cell culture can be cured by decontamination with the specific antibiotic regimen. Here, we describe the treatment of 35 mycoplasma-positive cell lines by the use of the novel antibiotic Mycozap(®) as well as evaluate its eradication performance versus the well-known routinely employed BM-Cyclins and fluoroquinolones molecules (175 treatments). Our data evidenced: i) the permanent elimination of mycoplasma infection by MycoZap(®), MRA, Enrofloxacin, Ciprofloxacin and BM-Cyclins in 46%, 29%, 40%, 43%, and 57% of the cultures, respectively; ii) a significant correlation between MRA and Ciprofloxacin eradication profile, as determined by the Spearman correlation coefficient (r = 0.3469, p < 0.05); iii) a mycoplasma eradication in 100% of cell lines by the exclusive adoption of MycoZap(®), Ciprofloxacin, Enrofloxacin, BM-Cyclin 1-2 antibiotic regimen, with the MRA exclusion; iv) the MycoZap(®) effectiveness even in case of a mycoplasmal load higher than 50 CFU/mL, as for SH-SY5Y and Neuro2A cells. In conclusion, we want to suggest an optimized antibiotic panel to get 100% mycoplasma-clearance especially in case of unique or treatment-resistant cellular models.

• Authors: Elisabetta Mariotti, Francesca D'Alessio, Peppino Mirabelli, Rosa Di Noto, Giuliana Fortunato, Luigi Del Vecchio
• Journal: Biologicals : journal of the International Association of Biological Standardization (Biologicals) Vol. 40 Issue 1 Pg. 88-91 (Jan 2012) ISSN: 1095-8320 [Electronic] England
• PMID: 22078664 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 The International Alliance for Biological Standardization. Published by Elsevier Ltd. All rights reserved.

Chemical References

• Anti-Infective Agents

Topics

• Animals
• Anti-Infective Agents (pharmacology)
• Cell Culture Techniques
• Cell Line, Tumor
• Humans
• Microbial Viability (drug effects)
• Mycoplasma
• Mycoplasma Infections (drug therapy)