Abstract |
Alkamides are fatty acid amides of wide distribution in plants, structurally related to N-acyl-L- homoserine lactones (AHLs) from Gram-negative bacteria and to N- acylethanolamines (NAEs) from plants and mammals. Global analysis of gene expression changes in Arabidopsis thaliana in response to N-isobutyl decanamide, the most highly active alkamide identified to date, revealed an overrepresentation of defense-responsive transcriptional networks. In particular, genes encoding enzymes for jasmonic acid (JA) biosynthesis increased their expression, which occurred in parallel with JA, nitric oxide (NO) and H₂O₂ accumulation. The activity of the alkamide to confer resistance against the necrotizing fungus Botrytis cinerea was tested by inoculating Arabidopsis detached leaves with conidiospores and evaluating disease symptoms and fungal proliferation. N-isobutyl decanamide application significantly reduced necrosis caused by the pathogen and inhibited fungal proliferation. Arabidopsis mutants jar1 and coi1 altered in JA signaling and a MAP kinase mutant (mpk6), unlike salicylic acid- (SA) related mutant eds16/sid2-1, were unable to defend from fungal attack even when N-isobutyl decanamide was supplied, indicating that alkamides could modulate some necrotrophic-associated defense responses through JA-dependent and MPK6-regulated signaling pathways. Our results suggest a role of alkamides in plant immunity induction.
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Authors | Alfonso Méndez-Bravo, Carlos Calderón-Vázquez, Enrique Ibarra-Laclette, Javier Raya-González, Enrique Ramírez-Chávez, Jorge Molina-Torres, Angel A Guevara-García, José López-Bucio, Luis Herrera-Estrella |
Journal | PloS one
(PLoS One)
Vol. 6
Issue 11
Pg. e27251
( 2011)
ISSN: 1932-6203 [Electronic] United States |
PMID | 22076141
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Arabidopsis Proteins
- Biomarkers
- Cyclopentanes
- Oxylipins
- Plant Growth Regulators
- Polyunsaturated Alkamides
- RNA, Messenger
- jasmonic acid
- Hydrogen Peroxide
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Topics |
- Arabidopsis
(drug effects, metabolism, microbiology)
- Arabidopsis Proteins
(genetics, metabolism)
- Biomarkers
(metabolism)
- Blotting, Western
- Botrytis
(pathogenicity)
- Cyclopentanes
(metabolism)
- Gene Expression Profiling
- Hydrogen Peroxide
(metabolism)
- Infection Control
- Oligonucleotide Array Sequence Analysis
- Oxylipins
(metabolism)
- Plant Diseases
(immunology, microbiology, prevention & control)
- Plant Growth Regulators
(metabolism)
- Plant Immunity
(drug effects)
- Plant Leaves
(drug effects, metabolism)
- Polyunsaturated Alkamides
(pharmacology)
- RNA, Messenger
(genetics)
- Real-Time Polymerase Chain Reaction
- Signal Transduction
(drug effects)
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