Screening and identification of a renal carcinoma specific peptide from a phage display peptide library.

Abstract

BACKGROUND:

Specific peptide ligands to cell surface receptors have been extensively used in tumor research and clinical applications. Phage display technology is a powerful tool for the isolation of cell-specific peptide ligands. To screen and identify novel markers for renal cell carcinoma, we evaluated a peptide that had been identified by phage display technology.

METHODS:

A renal carcinoma cell line A498 and a normal renal cell line HK-2 were used to carry out subtractive screening in vitro with a phage display peptide library. After three rounds of panning, there was an obvious enrichment for the phages specifically binding to the A498 cells, and the output/input ratio of phages increased about 100 fold. A group of peptides capable of binding specifically to the renal carcinoma cells were obtained, and the affinity of these peptides to the targeting cells and tissues was studied.

RESULTS:

Through a cell-based ELISA, immunocytochemical staining, immunohistochemical staining, and immunofluorescence, the Phage ZT-2 and synthetic peptide ZT-2 were shown to specifically bind to the tumor cell surfaces of A498 and incision specimens, but not to normal renal tissue samples.

CONCLUSION:

A peptide ZT-2, which binds specifically to the renal carcinoma cell line A498 was selected from phage display peptide libraries. Therefore, it provides a potential tool for early diagnosis of renal carcinoma or targeted drug delivery in chemotherapy.

• Authors: Xiangan Tu, Jintao Zhuang, Wenwei Wang, Liang Zhao, Liangyun Zhao, Jiquan Zhao, Chunhua Deng, Shaopeng Qiu, Yuanyuan Zhang
• Journal: Journal of experimental & clinical cancer research : CR (J Exp Clin Cancer Res) Vol. 30 Pg. 105 (Nov 10 2011) ISSN: 1756-9966 [Electronic] England
• PMID: 22071019 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Retracted Publication)

Chemical References

• Oligopeptides
• Peptide Library
• Peptides
• peptide ZT-2

Topics

• Amino Acid Sequence
• Binding, Competitive
• Carcinoma, Renal Cell (metabolism)
• Cell Line
• Enzyme-Linked Immunosorbent Assay
• Humans
• Kidney Neoplasms (metabolism)
• Oligopeptides (metabolism)
• Peptide Library
• Peptides (chemistry, metabolism)
• Protein Binding