Abstract | BACKGROUND: One of the well-defined and characterized protein modifications usually produced by oxidation is carbonylation, an irreversible non-enzymatic modification of proteins. However, carbonyl groups can be introduced into proteins by non-oxidative mechanisms. Reactive carbonyl compounds have been observed to have increased in patients with renal failure. In the present work we have described a procedure designed as aldehyde capture to calculate the protein carbonyl stress derived solely from lipid peroxidation. METHODS:
Acrolein- albumin adduct was prepared as standard at alkaline pH. Rat liver microsomal membranes and serum samples from patients with diabetic nephropathy were subjected to the aldehyde capture procedure and aldol- protein formation. Before alkalinization and incubation, samples were precipitated and redisolved in 6M guanidine. The absorbances of the samples were read with a spectrophotometer at 266 nm against a blank of guanidine. RESULTS: CONCLUSION: The aldehyde- protein adduct represents a non oxidative component of carbonyl stress, independent of the direct amino acid oxidation and could constitute a practical and novelty strategy to measure the carbonyl stress derived solely from lipid peroxidation and particularly in diabetic nephropathy patients. In addition, we are in a position to propose an alternative explanation of why alkalinization of urine attenuates rhabdomyolysis-induced renal dysfunction.
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Authors | Rafael Medina-Navarro, Renato Nieto-Aguilar, Cleto Alvares-Aguilar |
Journal | Lipids in health and disease
(Lipids Health Dis)
Vol. 10
Pg. 201
(Nov 07 2011)
ISSN: 1476-511X [Electronic] England |
PMID | 22059738
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Aldehydes
- Blood Proteins
- Serum Albumin
- NADP
- Acrolein
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Topics |
- Acrolein
(chemistry)
- Aldehydes
(blood, chemistry)
- Animals
- Blood Proteins
(metabolism)
- Case-Control Studies
- Diabetic Nephropathies
(blood, physiopathology)
- Humans
- Kinetics
- Lipid Peroxidation
- Liver
(enzymology)
- Male
- Microsomes
(enzymology)
- NADP
(chemistry)
- Protein Carbonylation
- Rats
- Rats, Sprague-Dawley
- Reference Standards
- Serum Albumin
(chemistry)
- Spectrophotometry
(standards)
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