Increased lymphocyte infiltration in patients with head and neck cancer treated with the IRX-2 immunotherapy regimen.

Abstract

Twenty-seven subjects with squamous cell cancer of the head and neck received the neoadjuvant IRX-2 immunotherapy regimen prior to surgery in a Phase 2 trial. Pretreatment tumor biopsies were compared with the primary tumor surgical specimens for lymphocyte infiltration, necrosis and fibrosis, using hematoxylin and eosin stain and immunohistochemistry in 25 subjects. Sections were examined by three pathologists. Relative to pretreatment biopsies, increases in lymphocyte infiltration (LI) were seen using H and E or immunohistochemistry. CD3+ CD4+ T cells and CD20+ B cells were primarily found in the peritumoral stroma and CD3+ CD8+ T cells and CD68+ macrophages were mainly intratumoral. LI in the surgical specimens were associated with reductions in the primary tumor size. Improved survival at 5 years was correlated with high overall LI in the tumor specimens. Neoadjuvant IRX-2 immunotherapy regimen may restore immune responsiveness presumably by mobilizing tumor infiltrating effector lymphocytes and macrophages into the tumor.

• Authors: Neil L Berinstein, Gregory T Wolf, Paul H Naylor, Lorraine Baltzer, James E Egan, Harvey J Brandwein, Theresa L Whiteside, Lynn C Goldstein, Adel El-Naggar, Cecile Badoual, Wolf-Herve Fridman, J Michael White, John W Hadden
• Journal: Cancer immunology, immunotherapy : CII (Cancer Immunol Immunother) Vol. 61 Issue 6 Pg. 771-82 (Jun 2012) ISSN: 1432-0851 [Electronic] Germany
• PMID: 22057678 (Publication Type: Clinical Trial, Phase II, Journal Article, Multicenter Study)

Chemical References

• Cytokines
• IRX 2

Topics

• Adult
• Aged
• Cytokines (administration & dosage, immunology)
• Female
• Head and Neck Neoplasms (immunology, pathology, therapy)
• Humans
• Immunotherapy
• Lymphocytes, Tumor-Infiltrating (immunology)
• Male
• Middle Aged
• Regression Analysis
• Survival Analysis