Calcium phosphate nanoparticles with an asymmetric lipid bilayer coating for siRNA delivery to the tumor.

Abstract

Calcium phosphate (CaP) nanoparticles (NP) with an asymmetric lipid bilayer coating have been designed for targeted delivery of siRNA to the tumor. An anionic lipid, dioleoylphosphatydic acid (DOPA), was employed as the inner leaflet lipid to coat the nano-size CaP cores, which entrap the siRNA, such that the coated cores were soluble in organic solvent. A suitable neutral or cationic lipid was used as the outer leaflet lipid to form an asymmetric lipid bilayer structure verified by the measurement of NP zeta potential. The resulting NP was named LCP-II with a size of about 25 to 30nm in diameter and contained a hollow core as revealed by TEM imaging. PEGylation of NP was done by including a PEG-phospholipid conjugate, with or without a targeting ligand anisamide, in the outer leaflet lipid mixture. The sub-cellular distribution studied in the sigma receptor positive human H460 lung cancer cells indicated that LCP-II could release more cargo to the cytoplasm than our previous lipid/protamine/DNA (LPD) formulation, leading to a significant (~40 fold in vitro and ~4 fold in vivo) improvement in siRNA delivery. Bio-distribution study showed that LCP-II required more PEGylation for MPS evasion than the previous LPD, probably due to increased surface curvature in LCP-II.

• Authors: Jun Li, Yang Yang, Leaf Huang
• Journal: Journal of controlled release : official journal of the Controlled Release Society (J Control Release) Vol. 158 Issue 1 Pg. 108-14 (Feb 28 2012) ISSN: 1873-4995 [Electronic] Netherlands
• PMID: 22056915 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
• Copyright: Copyright © 2011 Elsevier B.V. All rights reserved.

Chemical References

• Calcium Phosphates
• Fatty Acids, Monounsaturated
• Lipid Bilayers
• Phosphatidic Acids
• Phosphatidylcholines
• Quaternary Ammonium Compounds
• RNA, Small Interfering
• dioleoylphosphatidic acid
• calcium phosphate
• 1,2-oleoylphosphatidylcholine
• 1,2-dioleoyloxy-3-(trimethylammonium)propane

Topics

• Animals
• Calcium Phosphates (administration & dosage, chemistry)
• Cell Line, Tumor
• Fatty Acids, Monounsaturated (administration & dosage, chemistry)
• Female
• Gene Silencing
• Gene Transfer Techniques
• Humans
• Lipid Bilayers (administration & dosage, chemistry)
• Mice
• Mice, Nude
• Nanoparticles (administration & dosage, chemistry)
• Neoplasms (genetics, metabolism)
• Phosphatidic Acids (administration & dosage, chemistry)
• Phosphatidylcholines (administration & dosage, chemistry)
• Quaternary Ammonium Compounds (administration & dosage, chemistry)
• RNA, Small Interfering (administration & dosage, chemistry)