The antinociceptive efficacy of HWTX-I epidurally administered in rheumatoid arthritis rats.

Abstract

Rheumatoid arthritis (RA) is one of the inflammatory kinds of arthritis in the clinical situation, and cytosolic Ca2+ overload has been proposed as one of the primary factors for many inflammatory cells activation, which lead to relative enzymes and inflammatory factors release. It is therefore accepted that Ca2+ channel blockers can protect joint injury from inflammation. In the present study we investigated the possible molecular mechanism of the antinociceptive efficacy of HWTX-I, a spider peptide toxin blocking Ca2+ channels, on the rat rheumatoid arthritis model. Our study demonstrates that HWTX-I can relieve pain in the inflammatory joints and eliminate arthrocele to some degree. Moreover, HWTX-I can also decrease the concentration of tumour necrosis factor α (TNF-α) and increase the concentration of interleukin 4(IL-4) and interleukin 10(IL-10) in rat's serum. HWTX-I can also decrease the mRNA expression level of related factors of TNF-α, interleukin 1β (IL-1β) and interleukin 6(IL-6) in inflammatory pathways in rheumatoid arthritis. Therefore, the present results show that the epidural administration of HWTX-I is effective in antinociception in the rat model of rheumatoid arthritis, which may act through its inhibition on certain inflammatory pathways.

• Authors: Z Wen Tao, T Gu Yang, R Ying, W Mao Cai, L Lin, L Chi Miao, H Peng, C Joa Qin
• Journal: International journal of sports medicine (Int J Sports Med) Vol. 32 Issue 11 Pg. 869-74 (Nov 2011) ISSN: 1439-3964 [Electronic] Germany
• PMID: 22052031 (Publication Type: Journal Article)
• Copyright: Georg Thieme Verlag KG Stuttgart · New York.

Chemical References

• Analgesics
• Calcium Channel Blockers
• Calcium Channels
• Cytokines
• Neurotoxins
• Reptilian Proteins
• Spider Venoms
• huwentoxin I
• Calcium

Topics

• Analgesics (administration & dosage, pharmacology)
• Animals
• Arthritis, Experimental (drug therapy, physiopathology)
• Arthritis, Rheumatoid (drug therapy, physiopathology)
• Calcium (metabolism)
• Calcium Channel Blockers (administration & dosage, pharmacology)
• Calcium Channels (drug effects)
• Cytokines (drug effects, metabolism)
• Injections, Epidural
• Male
• Neurotoxins (administration & dosage, pharmacology)
• Pain (drug therapy, etiology)
• Rats
• Rats, Sprague-Dawley
• Reptilian Proteins (administration & dosage, pharmacology)
• Spider Venoms (administration & dosage, pharmacology)