Abstract | BACKGROUND: METHODS: We induced middle cerebral artery occlusion (MCAO) in male rats via intracranial injection of endothelin-1 (ET-1), a potent vasoconstrictor, and then treated with local delivery of pGSN. Whole brain laser Doppler perfusion imaging was performed through the skull to assess MCAO effectiveness. Cylinder and vibrissae tests evaluated sensorimotor function before and 72 h after MCAO. Infarct volumes were examined 72 h after MCAO via 2, 3, 5-triphenyltetrazolium chloride (TTC) assay. RESULTS: Estimates of relative cerebral perfusion were significantly decreased in all groups receiving MCAO with no differences detected between treatments. Despite equivalent initial strokes, the infarct volume of the pGSN treatment group was significantly reduced compared with the untreated MCAO rats at 72 h. ET-1 induced significant deficits in both cylinder and vibrissae tests while pGSN significantly limited these deficits. CONCLUSION:
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Authors | Huong T Le, Aaron C Hirko, Jeffrey S Thinschmidt, Maria Grant, Zhimin Li, Joanna Peris, Michael A King, Jeffrey A Hughes, Sihong Song |
Journal | Experimental & translational stroke medicine
(Exp Transl Stroke Med)
Vol. 3
Issue 1
Pg. 13
(Nov 02 2011)
ISSN: 2040-7378 [Electronic] England |
PMID | 22047744
(Publication Type: Journal Article)
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