Abstract |
Anti-ras p21 monoclonal antibody (RASK-3) was used for immunoscintigraphy of human cancer cell lines in nude mice. Iodine-125-labeled RASK-3 was injected into nude mice with either human colon cancers (FCC-1 or BM-314) or lung cancer (KNS-62). Clear images were obtained in all three cancers 7 days after the injection of antibody. No localization of 125I-labeled control monoclonal antibody was observed. The ratio of tissue/blood radioactivity and % ID/g in the tumor were significantly higher than other organs by Day 8. The specific localization index examined by 131I-RASK-3 and 125I-control monoclonal antibody was also higher in the tumor than in other tissues. In the in vitro study, binding of RASK-3 to tumor cells increased significantly by treatment of cells with either lysolecithin or periodate-lysine-paraformaldehyde, which confirmed the intracellular localization of ras p21. The mechanism by which anti-ras p21 antibodies accumulate in tumor sites could be the necrotic changes in tumor cells or changes in membrane permeability of non-necrotic cells. These results provide a strong rationale for the utilization of ras p21 as a target antigen in the imaging of a variety of human cancers.
|
Authors | Y Katoh, K Nakata, K Kohno, M Shima, A Satoh, Y Kusumoto, N Ishii, T Kohji, H Shiku, S Nagataki |
Journal | Journal of nuclear medicine : official publication, Society of Nuclear Medicine
(J Nucl Med)
Vol. 31
Issue 9
Pg. 1520-6
(Sep 1990)
ISSN: 0161-5505 [Print] United States |
PMID | 2203882
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Antibodies, Monoclonal
- Iodine Radioisotopes
- Oncogene Protein p21(ras)
|
Topics |
- Animals
- Antibodies, Monoclonal
- Colonic Neoplasms
(diagnostic imaging)
- Humans
- Iodine Radioisotopes
- Lung Neoplasms
(diagnostic imaging)
- Mice
- Mice, Nude
- Neoplasm Transplantation
- Oncogene Protein p21(ras)
(immunology)
- Radionuclide Imaging
|