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Immunoscintigraphy of human tumors transplanted in nude mice with radiolabeled anti-ras p21 monoclonal antibodies.

Abstract
Anti-ras p21 monoclonal antibody (RASK-3) was used for immunoscintigraphy of human cancer cell lines in nude mice. Iodine-125-labeled RASK-3 was injected into nude mice with either human colon cancers (FCC-1 or BM-314) or lung cancer (KNS-62). Clear images were obtained in all three cancers 7 days after the injection of antibody. No localization of 125I-labeled control monoclonal antibody was observed. The ratio of tissue/blood radioactivity and % ID/g in the tumor were significantly higher than other organs by Day 8. The specific localization index examined by 131I-RASK-3 and 125I-control monoclonal antibody was also higher in the tumor than in other tissues. In the in vitro study, binding of RASK-3 to tumor cells increased significantly by treatment of cells with either lysolecithin or periodate-lysine-paraformaldehyde, which confirmed the intracellular localization of ras p21. The mechanism by which anti-ras p21 antibodies accumulate in tumor sites could be the necrotic changes in tumor cells or changes in membrane permeability of non-necrotic cells. These results provide a strong rationale for the utilization of ras p21 as a target antigen in the imaging of a variety of human cancers.
AuthorsY Katoh, K Nakata, K Kohno, M Shima, A Satoh, Y Kusumoto, N Ishii, T Kohji, H Shiku, S Nagataki
JournalJournal of nuclear medicine : official publication, Society of Nuclear Medicine (J Nucl Med) Vol. 31 Issue 9 Pg. 1520-6 (Sep 1990) ISSN: 0161-5505 [Print] United States
PMID2203882 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antibodies, Monoclonal
  • Iodine Radioisotopes
  • Oncogene Protein p21(ras)
Topics
  • Animals
  • Antibodies, Monoclonal
  • Colonic Neoplasms (diagnostic imaging)
  • Humans
  • Iodine Radioisotopes
  • Lung Neoplasms (diagnostic imaging)
  • Mice
  • Mice, Nude
  • Neoplasm Transplantation
  • Oncogene Protein p21(ras) (immunology)
  • Radionuclide Imaging

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