Subunit approach to evaluation of the immune protective potential of leptospiral antigens.

Abstract

Leptospirosis is the most widespread zoonosis in the world. Current vaccines are based on whole-cell preparations that cause severe side effects and do not induce satisfactory immunity. In light of the leptospiral genome sequences recently made available, several studies aimed at identification of protective recombinant immunogens have been performed; however, few such immunogens have been identified. The aim of this study was to evaluate 27 recombinant antigens to determine their potential to induce an immune response protective against leptospirosis in the hamster model. Experiments were conducted with groups of female hamsters immunized with individual antigen preparations. Hamsters were then challenged with a lethal dose of Leptospira interrogans. Thirteen antigens induced protective immune responses; however, only recombinant proteins LIC10325 and LIC13059 induced significant protection against mortality. These results have important implications for the development of an efficacious recombinant subunit vaccine against leptospirosis.

• Authors: Samuel R Félix, Daiane D Hartwig, Ana Paula C Argondizzo, Éverton F Silva, Fabiana K Seixas, Amilton C P Seixas Neto, Marco A Medeiros, Walter Lilenbaum, Odir A Dellagostin
• Journal: Clinical and vaccine immunology : CVI (Clin Vaccine Immunol) Vol. 18 Issue 12 Pg. 2026-30 (Dec 2011) ISSN: 1556-679X [Electronic] United States
• PMID: 22030369 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Bacterial
• Bacterial Vaccines
• Protein Subunits
• Vaccines, Subunit
• Vaccines, Synthetic

Topics

• Animals
• Antigens, Bacterial (genetics, immunology)
• Bacterial Vaccines (administration & dosage, genetics, immunology)
• Cricetinae
• Disease Models, Animal
• Female
• Leptospira interrogans (immunology, pathogenicity)
• Leptospirosis (immunology, prevention & control)
• Protein Subunits (genetics, immunology)
• Survival Analysis
• Vaccines, Subunit (administration & dosage, genetics, immunology)
• Vaccines, Synthetic (administration & dosage, genetics, immunology)