Separation and identification of HSP-associated protein complexes from pancreatic cancer cell lines using 2D CN/SDS-PAGE coupled with mass spectrometry.

Abstract

Protein complexes are a cornerstone of many biological processes and together they form various types of molecular machinery. A broad understanding of these protein complexes is crucial for revealing and building models of protein function and regulation. Pancreatic cancer is a highly lethal disease which is difficult to diagnose at early stage and even more difficult to cure. In this study, we applied a gradient clear native gel system combined with subsequent second-dimensional SDS-PAGE to separate protein complexes from cell lysates of SW1990 and PANC-1 pancreatic cancer cell lines with different degrees of differentiation. Ten heat-shock-protein- (HSP-) associated protein complexes were separated and identified, and the differentially expressed proteins related to cancers were also found, such as HSP60, protein disulfide-isomerase A4 (ERp72), and transitional endoplasmic reticulum ATPase (TER ATPase).

• Authors: Zhiyun Zhao, Hui Liu, Xinli Wang, Xiaodong Wang, Zhili Li
• Journal: Journal of biomedicine & biotechnology (J Biomed Biotechnol) Vol. 2011 Pg. 193052 ( 2011) ISSN: 1110-7251 [Electronic] United States
• PMID: 22028587 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Heat-Shock Proteins
• Neoplasm Proteins

Topics

• Cell Line, Tumor
• Databases, Protein
• Electrophoresis, Polyacrylamide Gel (methods)
• Heat-Shock Proteins (isolation & purification, metabolism)
• Humans
• Neoplasm Proteins (isolation & purification, metabolism)
• Pancreatic Neoplasms (chemistry, metabolism)
• Protein Binding
• Protein Interaction Mapping (methods)
• Proteomics
• Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (methods)
• Tandem Mass Spectrometry (methods)