Abstract | BACKGROUND: METHODS:
Remifentanil was administered through a catheter in the tail vein of male Sprague-Dawley rats for 10 minutes (30 μg · kg(-1) · min(-1)), 30 minutes (0.1, 1, and 10 μg · kg(-1) · min(-1)), or 120 minutes (0.1, 1, 3, and 10 μg · kg(-1) · min(-1)). The von Frey test and a tail-flick test were performed, followed by ERK1/2 immunohistochemistry. We examined whether intrathecal preadministration of the mitogen-activated protein kinase inhibitor U0126 suppresses hyperalgesia. RESULTS:
Remifentanil had a dose-dependent antinociceptive effect that rapidly diminished. Ten- or 30-minute remifentanil infusion did not induce hyperalgesia. However, tail-flick latency and mechanical pain threshold after infusion termination were significantly lower in the 120-minute remifentanil administration group than those in the control group, regardless of dose. Hyperalgesia duration was no longer than 60 minutes. Significantly more phospho-ERK1/2-immunoreactive neurons in the superficial spinal dorsal horn were observed in the remifentanil 120-minute groups with hyperalgesia than in the 30-minute remifentanil groups without hyperalgesia, although U0126 did not suppress hyperalgesia. CONCLUSIONS:
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Authors | Ryosuke Ishida, Tetsuro Nikai, Tatsuya Hashimoto, Toshiko Tsumori, Yoji Saito |
Journal | Anesthesia and analgesia
(Anesth Analg)
Vol. 114
Issue 1
Pg. 224-9
(Jan 2012)
ISSN: 1526-7598 [Electronic] United States |
PMID | 22025495
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Analgesics, Opioid
- Piperidines
- Protein Kinase Inhibitors
- Mitogen-Activated Protein Kinase 1
- Mitogen-Activated Protein Kinase 3
- Remifentanil
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Topics |
- Analgesics, Opioid
(administration & dosage, toxicity)
- Animals
- Drug Administration Schedule
- Hyperalgesia
(chemically induced, enzymology, physiopathology)
- Infusions, Intravenous
- Male
- Mitogen-Activated Protein Kinase 1
(antagonists & inhibitors, metabolism)
- Mitogen-Activated Protein Kinase 3
(antagonists & inhibitors, metabolism)
- Pain Measurement
- Pain Threshold
(drug effects)
- Phosphorylation
- Piperidines
(administration & dosage, toxicity)
- Protein Kinase Inhibitors
(pharmacology)
- Rats
- Rats, Sprague-Dawley
- Reaction Time
(drug effects)
- Remifentanil
- Time Factors
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