Chromosomal aberrations in ovine lymphocytes exposed in vitro to tolylfluanid.

Abstract

Chromosomal aberrations have been used as important cytogenetic biomarkers to study the mutagenic effects of different chemicals in vivo and in vitro. Chromosomal aberrations were evaluated in cultures of sheep lymphocytes in vitro exposed to the fungicide tolylfluanid. Lymphocyte cultures from three donors were exposed to four different concentrations of fungicide (1.10(-4) M(.)L; 1.10(-5) M(.)L; 1.10(-6) M(.)L; 1 × 10(-7) M(.)L). Chromosomal analysis showed a significant (P = 0.018 and 0.038 respectively, Anova test, P < 0.05, Tukey test) increase in the frequency of aberrant cells (ABC) in cultures treated with the highest negative experimental concentrations of tolylfluanid (1.10(-4) M(.)L; 1.10(-5) M(.)L) compared to control. Significantly increased numbers of chromatid breaks (7.67 ± 0.58% against 1.67 ± 2.08%, P = 0.009, Anova test, P < 0.05, Tukey test) and chromatid gaps (7.67 ± 1.15% against 2.67 ± 0.58%, P = 0.003, Anova test, P < 0.05, Tukey test) were observed in ovine cultures treated with the highest experimental concentration of tolylfluanid (1.10(-4) M(.)L). Tolylfluanid induced also chromosomal exchanges (P = 0.038, and 0.016 respectively, Anova test, P < 0.05, Tukey test) in ovine cultures treated with the highest experimental concentrations of tolylfluanid (1.10(-4) M(.)L; 1.10(-5) M(.)L). The mitotic index has not shown any statistical differences between the various treatments and control groups. Our results suggest a significant genotoxic effect of tolylfluanid only at the highest concentration in sheep peripheral lymphocytes in vitro.

• Authors: Irena Sutiaková, Natália Kovalkovičová, Václav Sutiak
• Journal: Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes (J Environ Sci Health B) Vol. 47 Issue 1 Pg. 1-6 ( 2012) ISSN: 1532-4109 [Electronic] England
• PMID: 22022782 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Fungicides, Industrial
• Mutagens

Topics

• Animals
• Cells, Cultured
• Chromosome Aberrations (drug effects)
• Environmental Monitoring
• Fungicides, Industrial (toxicity)
• Lymphocytes (cytology, drug effects)
• Mitotic Index
• Mutagenicity Tests
• Mutagens (toxicity)
• Sheep