Cyclosporin a inhibits albumin synthesis in Huh7 cells.

Abstract

BACKGROUND/AIMS:

Hypoalbuminemia occurs frequently in renal transplant recipients immediately after renal transplantation. We studied the regulation of hepatic albumin synthesis by cyclosporin A (CsA) in Huh7 cells.

METHODS:

Huh7 cells were incubated with various concentrations of CsA for 4, 8, 16, and 24 hours. Albumin was measured in Huh7 cell-conditioned medium by sandwich enzyme-linked immunosorbent assay and Western blot. Albumin mRNA expression was analyzed by Northern blotting in CsA-treated cells.

RESULTS:

CsA (10(-7)-10(-4) M) inhibited albumin synthesis in Huh7 cells in a dose- dependent manner. A Western blot analysis for albumin in the conditioned medium released from CsA-treated (10(-7)-10(-5) M) cells also showed significant inhibition of albumin synthesis in a dose-dependent manner. Vehicle (olive oil) did not affect albumin synthesis. In contrast, a Northern blot analysis revealed no inhibition of albumin mRNA expression by CsA at any time point from 1-24 hours, indicating that the inhibition of albumin synthesis occurred at the translational level.

CONCLUSIONS:

Our results suggest that inhibition of hepatic albumin synthesis by high dose CsA contributes to the hypoalbuminemia in renal transplant recipients.

• Authors: Youn Joo Jeon, Yong Soo Kim
• Journal: The Korean journal of internal medicine (Korean J Intern Med) Vol. 26 Issue 3 Pg. 314-9 (Sep 2011) ISSN: 2005-6648 [Electronic] Korea (South)
• PMID: 22016592 (Publication Type: Journal Article)

Chemical References

• Culture Media, Conditioned
• Immunosuppressive Agents
• RNA, Messenger
• Serum Albumin
• Cyclosporine

Topics

• Blotting, Northern
• Blotting, Western
• Carcinoma, Hepatocellular (genetics, metabolism)
• Cell Line, Tumor
• Cell Survival (drug effects)
• Culture Media, Conditioned (metabolism)
• Cyclosporine (pharmacology, toxicity)
• Dose-Response Relationship, Drug
• Enzyme-Linked Immunosorbent Assay
• Gene Expression Regulation, Neoplastic (drug effects)
• Humans
• Hypoalbuminemia (chemically induced, metabolism)
• Immunosuppressive Agents (pharmacology, toxicity)
• Liver Neoplasms (genetics, metabolism)
• RNA, Messenger (metabolism)
• Serum Albumin (genetics, metabolism)
• Time Factors