Abstract |
Melatonin and its derivatives modulate the Plasmodium falciparum and Plasmodium chabaudi cell cycle. Flow cytometry was employed together with the nucleic acid dye YOYO-1 allowing precise discrimination between mono- and multinucleated forms of P. falciparum-infected red blood cell. The use of YOYO-1 permitted excellent discrimination between uninfected and infected red blood cells as well as between early and late parasite stages. Fluorescence intensities of schizont-stage parasites were about 10-fold greater than those of ring-trophozoite form parasites. Melatonin and related indolic compounds including serotonin, N-acetyl- serotonin and tryptamine induced an increase in the percentage of multinucleated forms compared to non-treated control cultures. YOYO-1 staining of infected erythrocyte and subsequent flow cytometry analysis provides a powerful tool in malaria research for screening of bioactive compounds.
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Authors | Desirée Cigaran Schuck, Ramira Yuri Ribeiro, Arthur A Nery, Henning Ulrich, Célia R S Garcia |
Journal | Cytometry. Part A : the journal of the International Society for Analytical Cytology
(Cytometry A)
Vol. 79
Issue 11
Pg. 959-64
(Nov 2011)
ISSN: 1552-4930 [Electronic] United States |
PMID | 22015733
(Publication Type: Journal Article)
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Copyright | Copyright © 2011 International Society for Advancement of Cytometry. |
Chemical References |
- Benzoxazoles
- Fluorescent Dyes
- Quinolinium Compounds
- Tryptamines
- 1,1'-((4,4,7,7-tetramethyl)-4,7-diazaundecamethylene)bis-4-(3-methyl-2,3-dihydro(benzo-1,3-oxazole)-2-methylidene)quinolinium
- Serotonin
- tryptamine
- Melatonin
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Topics |
- Animals
- Benzoxazoles
(analysis)
- Cell Cycle
- Erythrocytes
(drug effects, parasitology, pathology)
- Flow Cytometry
(methods)
- Fluorescence
- Fluorescent Dyes
(analysis)
- Humans
- Life Cycle Stages
(physiology)
- Malaria
(parasitology, pathology)
- Melatonin
(pharmacology)
- Plasmodium chabaudi
(cytology, drug effects, physiology)
- Plasmodium falciparum
(cytology, drug effects, physiology)
- Quinolinium Compounds
(analysis)
- Serotonin
(pharmacology)
- Staining and Labeling
(methods)
- Tryptamines
(pharmacology)
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