Cerium nitrate (CN) was used as a topical
antiseptic agent for the treatment of
burn wounds and found to reduce the number of anticipated death in
burn. This decreased
burn related mortality cannot be explained by the control of
wound infection alone. In the studies performed to elucidate the unexplained effects of CN treatment, it was shown that CN treatment reduced the alarm
cytokine levels, decreased leukocyte activation, reduced macromolecular leakage and finally
burn edema formation. We hypothesized that CN treatment prevents the conversion of the zone of stasis to progressive tissue
necrosis by decreasing leukocyte activation and reducing macromolecular leakage and
burn edema. This was investigated on a well-described
burn comb model in the rats. Fifty-four rats were randomly divided into control and CN treatment groups. Each rat in CN treatment group received 0.04 M CN bathing 30 min after
burn whereas rats in control group received 0.09% saline bathing. Viability of zone of stasis is assessed with (99 m)Tc-sestamibi scintigraphy. Nine rats in each group were scintigraphically evaluated at the 3rd and 7th day after
burn and remaining 9 rats had macroscopic and histological examination at the 21st day after
burn to confirm the scintigraphic results. In CN treatment groups, the scintigraphic uptake ratios were higher both at post
burn day 3rd and 7th when compared to that of control groups. This was statistically significant (p≤0.05). In the CN treatment group, the results of the average percentage of the re-epithelialization in the zone of stasis were higher than that of control groups. The difference between the groups was also statistically significant (p≤0.05). These results were accepted that CN treatment prevents progressive tissue
necrosis in the zone of stasis. This study further elucidates the unexplained effects of CN treatment on
burn.